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  • Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture.

Cost-Effective Cosmetic-Grade Hyaluronan Hydrogels for ReNcell VM Human Neural Stem Cell Culture.

Biomolecules (2019-09-25)
Weili Ma, Won Hyuk Suh
ABSTRACT

Hyaluronic acid (HA) is a polysaccharide polymer frequently used as a starting material to fabricate hydrogels, especially for recapitulating the brain's extracellular matrix (ECM) for in vitro neural stem cell (NSC) cultures. Here, we report the successful synthesis of a methacrylated HA (MeHA) polymer from an inexpensive cosmetic-grade hyaluronan starting material. The MeHA polymers synthesized from cosmetic-grade HA yielded similar chemical purity to those from pharmaceutical/research-grade HA reported in the literature. Crosslinked MeHA (x-MeHA) hydrogels were formed using radical polymerization which resulted in mechanical properties matching previously reported mechanical property ranges for enhanced neuronal differentiation of NSCs. We assessed cellular adhesion, spreading, proliferation, and stiffness-dependent neuronal differentiation properties of ReNcell VM human neural stem cells (hNSCs) and compared our results to studies reported in the literature (that utilized non-human and human pluripotent cell-derived NSCs).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methacrylic anhydride, contains 2,000 ppm topanol A as inhibitor, ≥98%
Sigma-Aldrich
ReNcell VM Human Neural Progenitor Cell LIne, ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.
Sigma-Aldrich
Donkey Anti-Mouse IgG Antibody, FITC conjugate, Species Adsorbed, Chemicon®, from donkey
Sigma-Aldrich
Donkey serum
Sigma-Aldrich
Epidermal Growth Factor Protein, Human recombinant, The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.