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  • Alphav and beta1 integrins regulate dynamic compression-induced proteoglycan synthesis in 3D gel culture by distinct complementary pathways.

Alphav and beta1 integrins regulate dynamic compression-induced proteoglycan synthesis in 3D gel culture by distinct complementary pathways.

Osteoarthritis and cartilage (2009-10-06)
D H Chai, E C Arner, D W Griggs, A J Grodzinsky
ABSTRACT

Our goal was to test the hypothesis that specific integrin receptors regulate chondrocyte biosynthetic response to dynamic compression at early times in 3D gel culture, during initial evolution of the pericellular matrix, but prior to significant accumulation of further-removed matrix. The study was motivated by increased use of dynamic loading, in vitro, for early stimulation of tissue engineered cartilage, and the need to understand the effects of loading, in vivo, at early times after implantation of constructs. Bovine articular chondrocytes were seeded in 2% agarose gels (15x10(6)cells/mL) and incubated for 18 h with and without the presence of specific integrin blockers (small-molecule peptidomimetics, function-blocking antibodies, and RGD-containing disintegrins). Samples were then subjected to a 24-h dynamic compression regime found previously to stimulate chondrocyte biosynthesis in 3D gel as well as cartilage explant culture (1 Hz, 2.5% dynamic strain amplitude, 7% static offset strain). At the end of loading, proteoglycan (PG) synthesis ((35)S-sulfate incorporation), protein synthesis ((3)H-proline incorporation), DNA content (Hoechst dye 33258) and total glycosaminoglycan (GAG) content (dimethyl methylene blue (DMMB) dye binding) were assessed. Consistent with previous studies, dynamic compression increased PG synthesis and total GAG accumulation compared to free-swelling controls. Blocking alphavbeta3 abolished this response, independent of effects on controls, while blocking beta1 abolished the relative changes in synthesis when changes in free-swelling synthesis rates were observed. This study suggests that both alphavbeta3 and beta1 play a role in pathways that regulate stimulation of PG synthesis and accumulation by dynamic compression, but through distinct complementary mechanisms.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10), ascites fluid, clone JB1A (J10), Chemicon®
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
100 μL
Estimated to ship on May 09, 2025
Details...
A recombinant, preservative-free antibody is available for your target. Try ZRB1230
CN¥6,364.93
Sigma-Aldrich
Anti-Integrin αV Antibody, clone P3G8, clone P3G8, Chemicon®, from mouse
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SKUPack SizeAvailabilityPriceQuantity
100 μg
Estimated to ship on May 09, 2025
Details...
A recombinant, preservative-free antibody is available for your target. Try ZMS1053
CN¥4,891.37