- A readily synthesized cyclic pyrrolysine analogue for site-specific protein "click" labeling.
A readily synthesized cyclic pyrrolysine analogue for site-specific protein "click" labeling.
Chemical communications (Cambridge, England) (2011-03-10)
Ziyang Hao, Yanqun Song, Shixian Lin, Maiyun Yang, Yujie Liang, Jing Wang, Peng R Chen
PMID21387054
ABSTRACT
A concise route was developed for the facile synthesis of a cyclic pyrrolysine analogue bearing an azide handle. Directed evolution enabled the encoding of this non-natural amino acid in both prokaryotic and eukaryotic cells, which offers a highly efficient approach for the site-specific protein labeling using click chemistry.