- Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe.
Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe.
Chemical communications (Cambridge, England) (2016-11-18)
Cheol Ho Heo, Myoung Ki Cho, Seunggun Shin, Tae Hyeon Yoo, Hwan Myung Kim
PMID27853757
ABSTRACT
Herein, we developed a ratiometric two-photon probe (BHS3-EGF), derived from a pH sensitive dye and epidermal growth factor (EGF), for real-time monitoring and quantitative analysis of acidic luminal pH values during endocytic pathway activity. Two-photon microscopy imaging with BHS3-EGF allows the quantitative analysis of pH distributions of single vesicles and their dynamics in receptor-mediated endocytosis in real-time.
MATERIALS