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Key Documents

Safety Information

RAW 264.7 Cell Line murine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 91062702, mouse blood, Macrophage

Synonym(s):

RAW264 7 Cells, RAW264.7 Cells

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About This Item

UNSPSC Code:
41106514

biological source

mouse blood

packaging

tube of 5 μg 91062702-DNA-5UG
vial of  cells 91062702-1VL

growth mode

Semi-adherent

karyotype

Not specified

morphology

Macrophage

products

Lysozyme

receptors

Immunoglobulin, complement

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

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Show Differences

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This Item
SML1147SML0891SML2779
ML-SA1 ≥95% (HPLC)

SML0627

ML-SA1

IQ-1 ≥95% (HPLC)

SML1147

IQ-1

L-152,804 ≥98% (HPLC)

SML0891

L-152,804

Tanaproget ≥98% (HPLC)

SML2779

Tanaproget

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (HPLC)

form

powder

form

powder

form

powder

form

powder

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

DMSO: 5 mg/mL, clear (warmed)

solubility

DMSO: 10 mg/mL, clear

solubility

DMSO: 5 mg/mL, clear (warmed)

solubility

DMSO: 2 mg/mL, clear

color

white to beige

color

yellow to orange

color

white to beige

color

white to beige

Cell Line Origin

Mouse monocyte macrophage

Cell Line Description

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

Application

Metabolic studies
RAW 264.7 cell line has been used to study the anti-inflammatory effects of pristimerin.[1] It has also been used to test the internalization and intracellular survival of Listeria monocytogenes.[2]

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Culture Medium

DMEM + 2mM Glutamine + 10% FBS / FCS (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. Remove the cells mechanically. Cells are semi-adherent, i.e. some cells grow in suspension, some loosely attach to the surface and others flattened out and attached to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. How do I handle Cell Line frozen cells upon arrival?

      Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

    5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

      If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

    6. How do I resuscitate/thaw frozen Cell Line cells?

      A protocol for thawing cells can be found in the ECACC handbook.

    7. What medium should I use when I thaw the Cell Line cells?

      The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

    8. What passage are my Cell Line cells?

      If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

    9. How many Cell Line cells are in the vial?

      Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

    10. Are my Cell Line cells mycoplasma tested?

      ECACC routinely tests all manufactured cell banks for mycoplasma.

    11. How to freeze down Cell Line cells to make a cell stock?

      A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

    12. Are the Cell Line cells tested for viral pathogens?

      HPA Cultures does not perform any viral testing on the cell lines.

    13. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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