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Hep G2 Cell Line human

85011430, hepatocyte carcinoma

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Synonym(s):
HepG2 Cells
UNSPSC Code:
41106514

biological source

human liver

growth mode

Adherent

karyotype

Modal no. 55

morphology

Epithelial

products

Prothrombin, antithromin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian hepatocyte carcinoma

Cell Line Description

The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.

Application

Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay.
Study of hepatocyte function and specific protein expression

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10
THO1: 9
TPOX: 8,9
vWA: 17

Culture Medium

EMEM (EBSS)+ 2mM Glutamine + 1% Non Essential Amino Acids NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. Until then growth occurs mainly in islands.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. How do I handle Cell Line frozen cells upon arrival?

    Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

  5. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

  6. How do I resuscitate/thaw frozen Cell Line cells?

    A protocol for thawing cells can be found in the ECACC handbook.

  7. What medium should I use when I thaw the Cell Line cells?

    The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

  8. What passage are my Cell Line cells?

    If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

  9. How many Cell Line cells are in the vial?

    Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

  10. Are my Cell Line cells mycoplasma tested?

    ECACC routinely tests all manufactured cell banks for mycoplasma.

  11. How to freeze down Cell Line cells to make a cell stock?

    A protocol for freezing down cells for cell stocks can be found in the ECACC manual.

  12. Are the Cell Line cells tested for viral pathogens?

    HPA Cultures does not perform any viral testing on the cell lines.

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