YEAST1
Yeast Transformation Kit
reagents for introducing plasmid DNA into yeast
Synonym(s):
lithium acetate yeast transformation
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About This Item
Product Name
Yeast Transformation Kit, reagents for introducing plasmid DNA into yeast
grade
Molecular Biology
technique(s)
transformation: suitable
shipped in
dry ice
storage temp.
−20°C
Quality Level
usage
kit sufficient for >100 standard transformations
Related Categories
Other Notes
- Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
- Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
- Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
- Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
- Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
Application
Biochem/physiol Actions
Features and Benefits
- Easy and ready-to-use
- Requires as little as 10 ng of plasmid DNA
- Flexibility for any strain of yeast
- Sufficient for over 100 standard transformations
General description
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。
Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
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Instructions
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