WGA2
GenomePlex® Complete Whole Genome Amplification (WGA) Kit
Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
Synonym(s):
Whole genome amplification kit
About This Item
Related Categories
General description
Application
- microarray analysis
- SNP analysis
- STR analysis
- DNA archiving
Features and Benefits
- Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs
- Nanograms of samples can be preserved at –20 °C for future use
- Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)
- Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy
- Whole-genome representation with no detectable allele bias
- Compatible with many downstream applications such as TaqMan® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis
Other Notes
Legal Information
Kit Components Also Available Separately
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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What is the major difference between WGA1 and WGA2 kits?
Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?
The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.
Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?
Using this kit to amplify DNA from a single cell is not recommended. We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application. WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.
Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?
When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20. Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.
Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?
WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Articles
aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.
aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.
Protocols
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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