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Merck
CN

R7385

Phosphoramidon disodium salt

powder, ≥97% (HPLC)

Synonym(s):

N-(α-Rhamno­pyranosyl­oxy­hydroxy­phosphinyl)-Leu-Trp disodium salt, N-(α-Rhamno­pyranosyl­phos­phono)-L-leucyl-L-tryptophan disodium salt

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About This Item

Empirical Formula (Hill Notation):
C23H32N3Na2O10P
CAS Number:
119942-99-3
Molecular Weight:
587.47
NACRES:
NA.77
PubChem Substance ID:
24278681
UNSPSC Code:
12352202
MDL number:
MFCD00077870
Beilstein/REAXYS Number:
8105669

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Product Name

Phosphoramidon disodium salt, ≥97% (HPLC)

Quality Level

200

InChI

1S/C23H34N3O10P.2Na/c1-11(2)8-16(26-37(33,34)36-23-20(29)19(28)18(27)12(3)35-23)21(30)25-17(22(31)32)9-13-10-24-15-7-5-4-6-14(13)15;;/h4-7,10-12,16-20,23-24,27-29H,8-9H2,1-3H3,(H,25,30)(H,31,32)(H2,26,33,34);;/q;2*+1/p-2/t12-,16-,17-,18-,19+,20+,23-;;/m0../s1

SMILES string

[Na+].[Na+].CC(C)C[C@H](NP([O-])(=O)O[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O)C(=O)N[C@@H](Cc2c[nH]c3ccccc23)C([O-])=O

InChI key

OQKHVXFOYFBMDJ-ODIUWQMJSA-L

biological source

Streptomyces sp.

assay

≥97% (HPLC)

form

powder

solubility

H2O: 50 mg/mL

storage temp.

−20°C

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Application

Identification of phosphoramidon as a metallo-endopeptidase inhibitor was used to characterize endothelin converting enzyme as a metallo-endopeptidase. Phosphoramidon increases the production of β-amyloid protein, but not amyloid precursor protein, indicating that abnormal amyloid processing in Alzheimer′s disease may be carried out by a metallo-endopeptidase.
Phosphoramidon disodium salt has been used:
  • as the neprilysin (NEP) inhibitor: in amyloid beta (Aβ) uptake and degradation
  • to study its effect on keratinase activity
  • to treat primary microglia from wild-type mice

Biochem/physiol Actions

Phosphoramidon can inhibit ZMPSTE24, an integral membrane protein zinc metalloprotease. It is capable of blocking thermolysin.
Phosphoramidon is a potent inhibitor of thermolysin and other metallo-endopeptidases. It trongly inhibits mammalian enkephalinase and it does not inhibit trypsin, papain, chymotrypsin or pepsin. It weakly inhibits collagenase.

General description

Phosphoramidon [N-(α-rhamnopyranosyl-oxyhydroxyphosphinyl)-Leu-Trp] is a competitive inhibitor of several soluble zinc metalloproteases. Phosphoramidate is produced naturally by Streptomyces tanashiensis.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
WXBF6432V
720409
720409
0000375068
0000369853

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Deacetylation of TFEB promotes fibrillar A beta degradation by upregulating lysosomal biogenesis in microglia
Bao J, et al.
Protein & cell, 7(6), 417-433 (2016)
SIRT1 facilitates amyloid beta peptide degradation by upregulating lysosome number in primary astrocytes
Li MZ, et al.
Neural Regeneration Research, 13(11), 2005-2005 (2018)
J R Wu-Wong et al.
Molecular pharmacology, 44(2), 422-429 (1993-08-01)
Endothelin (ET) is generated from prepro-ET by dibasic pair proteolysis, followed by specific proteolytic cleavage between Trp21 and Val22. Currently, intense research efforts are focused on the investigation of a metalloprotease-like ET-converting enzyme that is inhibited by phosphoramidon but not
Thermolysin and Related Bacillus Metallopeptidases
Handbook of Proteolytic Enzymes, 1, 540-553 (2013)
Smart cleaning properties of a multi tolerance keratinolytic protease from an extremophilic Bacillus tequilensis hsTKB2: prediction of enzyme modification site
Paul T, et al.
Waste and biomass valorization, 5(6), 931-945 (2014)
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