Skip to Content

尊敬的客户：

目前国际形势复杂多变，关税政策尚不明朗，这可能对我们的产品价格产生一定影响。在此情况下，我们希望就订单事宜与您进行友好沟通。

基于当前的不确定性，如果您选择在此期间下单，我们将保留根据实际情况调整价格的权利。同时，我们也理解市场变化可能给您带来的困扰，因此如果在订单实际发货前因关税政策变动导致价格出现较大波动，默克将与您进行协商讨论并视情况对订单进行调整或取消。

Merck
CN
All Photos(4)

Key Documents

View All Documentation

Safety Information

PLN350

Sigma-Aldrich

GenElute™ Plasmid Miniprep Kit

greener alternative

sufficient for 350 purifications

Synonym(s):

GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

Sign Into View Organizational & Contract Pricing

Select a Size

100 G
CN¥758.56
250 G
CN¥1,541.14

CN¥758.56

Estimated to ship onMay 06, 2025Details

All Photos(4)

Select a Size

Change View
100 G
CN¥758.56
250 G
CN¥1,541.14

About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

CN¥758.56

Estimated to ship onMay 06, 2025Details

usage

sufficient for 350 purifications

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

greener alternative category

Aligned,

storage temp.

15-25°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
285740100684PHR1419
Sulfanilic acid GR for analysis ACS,Reag. Ph Eur

100686

Sulfanilic acid

磺胺酸 JIS special grade, 99.0-100.5%

285740

磺胺酸

Sulfanilic acid for elemental analysis

100684

Sulfanilic acid

对氨基苯磺酸 Pharmaceutical Secondary Standard; Certified Reference Material

PHR1419

对氨基苯磺酸

grade

ACS reagent

grade

JIS special grade

grade

-

grade

certified reference material, pharmaceutical secondary standard

assay

≥99.0-102.0% (acidimetric)

assay

99.0-100.5%

assay

-

assay

-

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

300

agency

reag. Ph. Eur.

agency

-

agency

-

agency

traceable to USP 1633506

form

solid

form

powder

form

solid

form

-

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

storage temp.

2-30°C

General description

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
The kit has been used for the purification of plasmid DNA of Dirofilaria repens.[1]

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

Regulatory Information

动植物源性产品
危险化学品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000434221
    0000434221
    0000427547
    0000427547
    0000401735

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).
    Mateju J
    BMC Infectious Diseases, 16, 171-171 (2016)
    Richard A Urbanowicz et al.
    Cell, 167(4), 1079-1087 (2016-11-05)
    The 2013-2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the co-circulation of several viral
    Simeon S Andrews et al.
    The Journal of biological chemistry, 294(30), 11549-11558 (2019-06-12)
    Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The
    Mahmood Faraz et al.
    The Journal of biological chemistry, 293(9), 3421-3435 (2018-01-11)
    Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is a tumor suppressor and a negative regulator of several receptor tyrosine kinases. The molecular mechanisms by which LRIG1 mediates its tumor suppressor effects and regulates receptor tyrosine kinases remain incompletely understood. Here
    Vanessa Meier-Stephenson et al.
    The Journal of biological chemistry, 296, 100589-100589 (2021-03-29)
    Approximately 250 million people worldwide are chronically infected with the hepatitis B virus (HBV) and are at increased risk of developing cirrhosis and hepatocellular carcinoma. The HBV genome persists as covalently closed circular DNA (cccDNA), which serves as the template
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service