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PFM250
GenElute™ Five-Minute Plasmid Miniprep Kit
sufficient for 250 purifications
Synonym(s):
GenElute™ Plasmid Miniprep Kit, Gen Elute
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
To inquire about this product PFM250, please contact your local Merck office or dealer. Contact Technical Service
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usage
sufficient for 250 purifications
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This Item | O4387 | MBD6009 | 11004760001 |
|---|---|---|---|
| form liquid | form solution | form suspension | form - |
| usage 0.1 mL sufficient for 100 RT-PCR reactions | usage 0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20) | usage - | usage sufficient for 50 labeling reactions |
| concentration 50 μM in H2O | concentration 70 μM in H2O | concentration - | concentration - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
General description
An overnight recombinant E. coli culture in LB (Luria-Bertani) broth is treated briefly (1-2 minutes) with a lysis reagent for rapid cell lysis and RNA degradation, without prior removal of culture medium. A binding solution is then mixed with the lysate, and DNA is captured on a silica-based binding column. Impurities are removed by a wash solution and bound plasmid DNA is then eluted in Tris buffer or water, ready for immediate use in various downstream applications. Typical yields of high copy plasmids are 2-6 mg from 400 mL of overnight culture in LB broth.
The GenElute Five-Minute Plasmid Miniprep Kit features an ultra-streamlined protocol resulting in high-quality plasmid DNA in about five minutes.
Recombinant E. coli overnight culture in LB (Luria-Bertani) broth is treated briefly (1-2 minutes) with a lysis reagent to effect rapid cell lysis and RNA degradation, without prior removal of culture medium. A binding solution is then mixed with the lysate, and DNA is captured on a silica-based binding column. Impurities are removed by a wash solution and bound plasmid DNA is then eluted in Tris buffer or water, ready for immediate use in various downstream applications. Typical yields of high copy plasmids are 2-6 μg from 400 μL of overnight culture in LB broth.
The GenElute Five-Minute Plasmid Miniprep Kits utilize a novel lysis procedure that eliminates the need to pellet cells from an overnight culture, remove growth media or clear a neutralized lysate. 400 μl of culture is added to a 2 mL collection tube and cells are lysed for 1−2 minutes. A Binding Solution is then added and the entire mixture is passed through a binding column. After washing the column to remove trace contaminants, the plasmid DNA is eluted in 10 mM Tris HCl, pH 8.5. Molecular biology water may be substituted as an elution reagent.
Recombinant E. coli overnight culture in LB (Luria-Bertani) broth is treated briefly (1-2 minutes) with a lysis reagent to effect rapid cell lysis and RNA degradation, without prior removal of culture medium. A binding solution is then mixed with the lysate, and DNA is captured on a silica-based binding column. Impurities are removed by a wash solution and bound plasmid DNA is then eluted in Tris buffer or water, ready for immediate use in various downstream applications. Typical yields of high copy plasmids are 2-6 μg from 400 μL of overnight culture in LB broth.
The GenElute Five-Minute Plasmid Miniprep Kits utilize a novel lysis procedure that eliminates the need to pellet cells from an overnight culture, remove growth media or clear a neutralized lysate. 400 μl of culture is added to a 2 mL collection tube and cells are lysed for 1−2 minutes. A Binding Solution is then added and the entire mixture is passed through a binding column. After washing the column to remove trace contaminants, the plasmid DNA is eluted in 10 mM Tris HCl, pH 8.5. Molecular biology water may be substituted as an elution reagent.
Application
GenElute™ Five-Minute Plasmid Miniprep Kit has been used in the isolation of plasmid.[1]
The GenElute Five-Minute Plasmid Miniprep Kit is intended for rapid preparation of plasmid DNA for use in capillary DNA sequencing, clone screening, restriction digestion, and PCR. For low copy plasmids, the kit can be used to prepare plasmid DNA for use in restriction digestion and PCR.
Features and Benefits
- No pelleting cells or clearing lysates
- Binding column works with any standard laboratory vacuum manifold
- Up to 5 μg of plasmid DNA or enough for 15 sequencing reactions
- DNA suitable for immediate capillary DNA sequencing
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1C - STOT SE 3
Target Organs
Central nervous system
Supplementary Hazards
Storage Class Code
3 - Flammable liquids
Flash Point(F)
69.8 °F
Flash Point(C)
21 °C
Regulatory Information
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Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB
Ma Q, et al.
Oncology Letters, 14(2), 1939-1942 (2017)
Effect of TERT on the growth of fibrosarcoma via caspase-3, survivin and PKB
Qiuye Ma
Oncology Letters (2017)
Qiuye Ma et al.
Oncology letters, 14(2), 1939-1942 (2017-08-10)
The present study explored the effect of telomerase reverse transcriptase (TERT) on the growth and apoptosis of fibrosarcoma, and investigated the potential molecular signalling pathways underlying its effect. A plasmid was constructed in order to overexpress TERT and siRNA was
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