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P5493

Sigma-Aldrich

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Synonym(s):

PBS

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About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, 10× concentrate, BioPerformance Certified, suitable for cell culture

grade

BioPerformance Certified
for molecular biology

Quality Level

100
200

sterility

0.2 μm filtered

form

solution

storage condition

(Tightly closed)

technique(s)

cell culture | mammalian: suitable
immunocytochemistry: suitable
immunofluorescence: suitable

impurities

Bioburden, tested
endotoxin, tested
≤5 ppm heavy metals (as Pb)

color

colorless

pH

7.2-7.6 (25 °C)

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for
suitable for flow cytometry
suitable for microscopy

application(s)

advanced drug delivery

foreign activity

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

SMILES string

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI key

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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Application

Phosphate buffered saline has been used:
  • as a cell wash solution in human dental pulp stem cells (DPSCs)
  • for perfusion studies in dissected brain samples
  • as wash solution for neurons culture cells sample preparation for immunofluorescence and immunocytochemical labelling
  • as wash solution for ovary and sperm during in vitro culture and fertilization

Packaging

4L package size comes in a box with a spigot for spill free dispensing.

Other Notes

When diluted to a 1X concentration, this product will yield a phosphate buffered saline solution with a phosphate buffer concentration of 0.01M and a sodium chloride concentration of 0.154M. The solution pH will be 7.4.
For additional information on our range of Biochemicals, please complete this form.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P5493, Phosphate buffered saline (PBS), contain calcium and magnesium?

    No, it does not contain calcium and magnesium.

  4. What is the concentration of the 10X stock solution of Product P5493, Phosphate buffered saline (PBS)?

    As formulated, the 10X solution is 100 mM phosphate and 1.54 M sodium chloride.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Differences in serum steroid hormones concentrations in spontaneously hypertensive
Kozlowska A, et al.
Physiological Research (2018)
Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media
Saif AH, et al.
The eurasia proceedings of science, technology, engineering & mathematics., 3(9), 181-188 (2018)
Stephen J Bush et al.
G3 (Bethesda, Md.), 9(2), 359-373 (2018-12-12)
One of the most significant physiological challenges to neonatal and juvenile ruminants is the development and establishment of the rumen. Using a subset of RNA-Seq data from our high-resolution atlas of gene expression in sheep (Ovis aries) we have provided
Diverse effect of BMP-2 homodimer on mesenchymal progenitors of different origin
Hrubi E, et al.
Human Cell : Official Journal of Human Cell Research Society, 31(2), 139-148 (2018)
Distribution and Neurochemistry of the Porcine Ileocaecal Valve Projecting Sensory Neurons in the Dorsal Root Ganglia and the Influence of Lipopolysaccharide from Different Serotypes of Salmonella spp. on the Chemical Coding of DRG Neurons in the Cell Cultures
Mikolajczyk A, et al.
International Journal of Molecular Sciences, 19(9), 2551-2551 (2018)

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