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P4832

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 150,000-300,000, solution, 0.01%, suitable for cell culture

Synonym(s):

PLL Solution

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About This Item

CAS Number:
25988-63-0
MDL number:
MFCD00165424
UNSPSC Code:
12352202
eCl@ss:
32160406
NACRES:
NA.75

product name

Poly-L-lysine solution, mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

Quality Level

200

product line

BioReagent

form

solution

mol wt

150,000-300,000

packaging

pkg of 50 mL

concentration

0.01%

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: soluble

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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General description

L-lysine is a homopolypeptide having two enantiomeric forms, L and D lysine. Through its three reactive groups (the carboxylic acid and the α and ε amines), L-lysine has the capacity to generate various branched polymeric structures, including dendrimers, branched polylysine, and hyperbranched polylysine (PLL). Poly-L-lysine is a polymer made up of positively charged amino acid residues, with each lysine residue typically containing one HBr.

Application

Poly-L-lysine has been used:

  • In coating 96-well plates for cell attachment in testing respiratory burst activity.
  • In coating glass cover slips for immunofluorescent labeling of intracellular structures.
  • For pretreating 6-well plate in myosatellite cell isolation protocol.
  • In coating coverslips for cell adhesion in E14 neuron culture to study possible mechanism of action of circRNA_01477 in neurons.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly‐l‐lysine (PLL) acts as polycationic polymers that have been extensively used to carry drugs, genes etc. across the cell membrane. Poly-L-lysine (PLL) has the capability to engage in the formation of polyelectrolyte complexes (PECs) by interacting with anionic entities such as polyanions, nucleic acid chains and some drugs.
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modulating the poly-L-lysine structure through the control of the protonation-deprotonation state of L-lysine
Luigi Stagi, et al.
Scientific Reports, 12(1), 19719-19719 (2022)
Effects of sex steroids on indices of protein turnover in rainbow trout (Oncorhynchus mykiss) white muscle
Cleveland BM and Weber GM
General and Comparative Endocrinology, 174(2), 132-142 (2011)
Molecular mechanisms underlying oxytocin-induced cardiomyocyte protection from simulated ischemia?reperfusion
Gonzalez-Reyes A, et al.
Molecular and Cellular Endocrinology, 412, 170-181 (2015)
Modulating the poly-L-lysine structure through the control of the protonation?deprotonation state of L-lysine
Stagi L, et al.
Scientific Reports (2022)
Caitlin N Spaulding et al.
eLife, 7 (2018-01-19)
Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to cause UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides a structural scaffold for
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Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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