P4557
Phenol solution
Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
Synonym(s):
Tris-equilabrated Phenol
About This Item
grade
for molecular biology
Quality Level
product line
BioReagent
Assay
≥99.0% (GC)
form
liquid
pH
7.7-8.1 (25 °C)
storage temp.
2-8°C
SMILES string
Oc1ccccc1
InChI
1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
InChI key
ISWSIDIOOBJBQZ-UHFFFAOYSA-N
Gene Information
human ... GABRA1(2554)
Looking for similar products? Visit Product Comparison Guide
General description
Application
- as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
- for extraction of DNA from Arabidopsis thaliana Rosette leaves
- in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
- as a component of extraction buffer to extract RNA from fruit pulp
- for the extraction of protein from roots
- for the isolation of fecal DNA
- for the extraction of caged RNA
Biochem/physiol Actions
Components
- 1 bottle Tris-equilabrated Phenol (product number P4557)
- Equilibration Buffer (product B5658)
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
Target Organs
Nervous system,Kidney,Liver,Skin
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the molarity of Phenol solution, Product P4557?
The Molarity is ~10.6. The density of the phenol is 1.05g/ml, and the molecular weight is 94.11.
I see that there are two layers in the bottle Phenol solution, Product P4557. Which layer do I use?
The top phase is Tris buffer, so use the lower/bottom phase.
What do I do to equilibrate the Phenol solution, Product P4557, to around pH 8?
This phenol solution (Product No. P4557) includes a separate bottle of equilibration buffer (Product No. B5658) to adjust the phenol phase to pH 7.9±0.2. After addition of the buffer to the phenol, it may take about 6 hours (mixture can be kept at 2-8 °C) until the bottom phase (phenol) becomes clear. Some of the haziness in the phenol phase may be due to some air bubbles caused by mixing by inversion. There could be a hazy interface between the phenol and aqueous phase which remains after the separation. The entire contents of the bottle of the buffer should be added to the phenol. If buffering less than the complete bottle, use at a ratio of 6.5 mL buffer to 100 mL phenol. For 10 mL of phenol, use 650 μL of buffer. For 1 mL of phenol, use 65 μL of buffer.
What is the final pH of Phenol solution, Product P4557, after adding the buffer?
The pH is typically 8.0; the specifications state a range of 7.7-8.1.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service