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Merck
CN

P2508

Proflavine hemisulfate salt hydrate

powder

Synonym(s):

3,6-Diaminoacridine hemisulfate salt

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About This Item

Empirical Formula (Hill Notation):
C13H11N3 · 0.5 H2SO4 · xH2O
CAS Number:
1811-28-5
Molecular Weight:
258.29 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24898329
EC Number:
217-320-3
Beilstein/REAXYS Number:
3921806
MDL number:
MFCD00149650

Key Documents

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Product Name

Proflavine hemisulfate salt hydrate, powder

InChI key

DTRLDKDFCPUGCA-UHFFFAOYSA-N

InChI

1S/2C13H11N3.H2O4S.H2O/c2*14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;1-5(2,3)4;/h2*1-7H,14-15H2;(H2,1,2,3,4);1H2

SMILES string

[H]O[H].OS(O)(=O)=O.Nc1ccc2cc3ccc(N)cc3nc2c1.Nc4ccc5cc6ccc(N)cc6nc5c4

form

powder

solubility

H2O: 10 mg/mL

fluorescence

λex 444 nm; λem 508 nm (0.01M HCl)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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General description

Proflavine is a small, amphipathic, acridine-derived fluorescent dye.Proflavine hemisulfate is used as a rapid stain for cytological studies of biological specimens. It stains cytoplasmic structures and nuclei. Proflavine has the capability to intercalate double stranded DNA.

Application

Proflavine hemisulfate salt hydrate has been used to stain cells of the 1483 cell line. It has also been used as a calibrating dye in fluorescence resonance energy transfer (FRET) analysis.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR5975
SHBN8862
SHBN0829
SHBL5759
MKCC6558

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Improving spatial resolution of a fiber bundle optical biopsy system
Matthew Kyrish
Proceedings of SPIE (2010)
Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy
M. R. Tadross
Journal of Microscopy (2009)
Emma V Ainsworth et al.
Chembiochem : a European journal of chemical biology, 17(24), 2324-2333 (2016-09-30)
The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar-assisted chemical synthesis. In Shewanella oneidensis MR-1 (MR-1), trans-outer-membrane electron transfer is
Proflavine Hemisulfate as a Fluorescent Contrast Agent for Point-of-Care Cytology
Sandra P. Prieto
PLoS ONE, 10(5) (2015)
A Orth et al.
Science advances, 5(4), eaav1555-eaav1555 (2019-04-30)
Optical fiber bundle microendoscopes are widely used for visualizing hard-to-reach areas of the human body. These ultrathin devices often forgo tunable focusing optics because of size constraints and are therefore limited to two-dimensional (2D) imaging modalities. Ideally, microendoscopes would record

Protocols

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