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Safety Information

P2192

Sigma-Aldrich

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

Synonym(s):

PCR Buffer Solution, PCR Reaction Buffer

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1 MG
CN¥669.75
5 MG
CN¥2,560.22
10 MG
CN¥4,050.78

CN¥669.75

Estimated to ship onJuly 02, 2025Details

New, lower price on this item!
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1 MG
CN¥669.75
5 MG
CN¥2,560.22
10 MG
CN¥4,050.78

About This Item

UNSPSC Code:
41106306
NACRES:
NA.52

CN¥669.75

Estimated to ship onJuly 02, 2025Details

New, lower price on this item!

Quality Level

300

form

liquid

packaging

vial of 1.5 mL

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

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ChIPAb+ CTCF - ChIP经验证的抗体和引物组

species reactivity

human, mouse

species reactivity

human, Xenopus

species reactivity

human, zebrafish, mouse, Xenopus, chicken, fish

species reactivity

human

clone

monoclonal

clone

monoclonal

clone

monoclonal

clone

1.2.1.5.4, monoclonal

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, dot blot: suitable, western blot: suitable

technique(s)

ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ChIP: suitable, ELISA: suitable, dot blot: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, multiplexing: suitable, western blot: suitable

Gene Information

human ... TCF7L2(6934)

Gene Information

-

Gene Information

human ... TBP(6908)

Gene Information

human ... CTCF(10664)

Application

10X PCR Buffer has been used as a component of the PCR mixture:
  • for the amplification of viral DNA[1]
  • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals[2]
  • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.[3]

Features and Benefits

  • Standalone buffer
  • Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Components

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000432738
    0000432738
    0000306660
    0000306650
    0000306660

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    G J Breedveld et al.
    Neurology, 59(4), 579-584 (2002-08-28)
    Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
    Maike Stam et al.
    Genetics, 162(2), 917-930 (2002-10-26)
    Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
    Ákos Boros et al.
    PloS one, 6(12), e29145-e29145 (2011-12-24)
    A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
    Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
    Thao N T P, et al.
    Open Journal of Genetics, 2014 (2014)
    Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
    Adewumi G A, et al.
    Food Biotechnology, 28(3), 250-268 (2014)
    View All Related Papers

    Protocols

    Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

    Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service