NA2120
GenElute™ Bacterial Genomic DNA Kits
sufficient for 350 purifications
Synonym(s):
Bacterial Genomic DNA, bacterial DNA prep, Gen Elute
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.55
usage
sufficient for 350 purifications
Quality Level
technique(s)
DNA purification: suitable
storage temp.
15-25°C
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General description
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Application
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
The purified bacterial genomic DNA is ready for downstream applications such as:
GenElute™ Bacterial Genomic DNA Kits has been used:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
GenElute™ Bacterial Genomic DNA Kits has been used:
- to isolate DNA
- to prepare DNA from E. coli strain
- to extract genomic DNA
Features and Benefits
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
Principle
The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system, Respiratory system
Flash Point(F)
closed cup
Flash Point(C)
closed cup
Regulatory Information
危险化学品
常规特殊物品
Certificates of Analysis (COA)
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Nora Vázquez-Laslop et al.
Journal of bacteriology, 188(10), 3494-3497 (2006-05-05)
Bacterial populations contain persisters, cells which survive exposure to bactericidal antibiotics and other lethal factors. Persisters do not have a genetic resistance mechanism, and their means to tolerate killing remain unknown. In exponentially growing populations of Escherichia coli the frequency
Margaret T Ho et al.
Genome announcements, 5(24) (2017-06-18)
We report here the complete assembled genome sequence of Acinetobacter calcoaceticus CA16, which is capable of utilizing diesel and lignin as a sole carbon source. CA16 contains a 4,110,074-bp chromosome and a 5,920-bp plasmid. The assembled sequences will help elucidate
Ikrema Hassan et al.
Genome announcements, 4(1) (2016-01-16)
Here we report the complete genomic sequence of the bacterium Arthrobacter sp. strain LS16, consisting of a single circular chromosome of 3.85 Mb with no identified plasmid. Data contained within will facilitate future genetic modification and engineering of the Arthrobacter sp.
Urinary tract Infection Escherichia coli is related to the environmental Escherichia coli in their DNA barcoding and antibiotic resistance patterns in Grenada
Farmer K, et al.
Advances in Microbiology, 6(01), 33-33 (2016)
Rajalakshmi Srinivasan et al.
Nucleic acids research, 43(1), 295-308 (2014-11-29)
The gene expression state of exponentially growing Escherichia coli cells is manifested by high expression of essential and growth-associated genes and low levels of stress-related and horizontally acquired genes. An important player in maintaining this homeostasis is the H-NS-StpA gene
Protocols
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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