M3770
Micrococcus lysodeikticus ATCC No. 4698
suitable for substrate for the assay of lysozyme, lyophilized cells
Synonym(s):
Micrococcus luetus
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
form
lyophilized cells
Quality Level
suitability
suitable for substrate for the assay of lysozyme
storage temp.
−20°C
Application
Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles.
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes.
Biochem/physiol Actions
Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.
Analysis Note
Contains polynucleotide phosphorylase.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
高风险级别生物产品--病毒，疫苗等
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Generation of a reference transcriptome for evaluating rainbow trout responses to various stressors.
Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Jingwei Xie et al.
Journal of colloid and interface science, 317(2), 469-476 (2007-10-20)
A co-axial electrospray process was developed to encapsulate protein-based drugs in biodegradable polymeric microparticles eliminating the key problem faced by other conventional methods of protein encapsulation--the primary emulsion being a major cause for protein denaturation and aggregation. Bovine serum albumin
Ghosh et al.
Biochemical engineering journal, 6(1), 19-24 (2000-07-26)
This paper discusses the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration (30kDa MWCO, polysulphone membrane). Lysozyme is preferentially transmitted through the membrane while the membrane largely retains other egg white proteins. Improvement in system hydrodynamics resulted in
Ingrid Bourgeois et al.
FEMS microbiology letters, 290(1), 105-113 (2008-11-26)
The nucleotide sequence of atlL, a gene encoding a putative Staphylococcus lugdunensis peptidoglycan hydrolase, was determined using degenerate consensus PCR and genome walking. This 3837-bp gene encodes a protein, AtlL, that appears as a putative bifunctional autolysin with a 29-amino
Protocols
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。
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