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M3770

Micrococcus lysodeikticus ATCC No. 4698

suitable for substrate for the assay of lysozyme, lyophilized cells

Synonym(s):

Micrococcus luetus

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
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form

lyophilized cells

Quality Level

200

suitability

suitable for substrate for the assay of lysozyme

storage temp.

−20°C

Application

Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles.
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes.

Biochem/physiol Actions

Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.

Analysis Note

Contains polynucleotide phosphorylase.

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

高风险级别生物产品--病毒，疫苗等

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Certificates of Analysis (COA)

Lot/Batch Number
0000522923
0000522923
0000480950
0000480950
0000480949

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Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Jingwei Xie et al.
Journal of colloid and interface science, 317(2), 469-476 (2007-10-20)
A co-axial electrospray process was developed to encapsulate protein-based drugs in biodegradable polymeric microparticles eliminating the key problem faced by other conventional methods of protein encapsulation--the primary emulsion being a major cause for protein denaturation and aggregation. Bovine serum albumin
View All Related Papers

Global Trade Item Number

SKUGTIN
M3770-25G04061834050582
M3770-100G04061834050575
M3770-5G04061835506415