L6037
Escort™ II Transfection Reagent
Lipid formulation for nucleic acid transfections
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About This Item
UNSPSC Code:
12352200
grade
for molecular biology
form
dried film
usage
1.5 mL sufficient for 150 transfections
storage temp.
2-8°C
General description
Escort™ II is a unique formulation of a proprietary cationic lipid and the neutral lipid DOPE. This liposome-forming compound is used for transfection of nucleic acids into eukaryotic cells.
Application
Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 25 μl Escort™ II and 5 μg DNA (in provided unique DNA Dilution buffer) per 6 cm cell culture plate. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Escort™ II:
B16-F0
BHK-21
CHO-K1
COS-1
COS-7
CV-1
D. mel (S2)
F9
HepG2
HEK293
HeLa
HUVEC
K562
L929
MDCK
NIH3T3
P19
PC-12
Vero
B16-F0
BHK-21
CHO-K1
COS-1
COS-7
CV-1
D. mel (S2)
F9
HepG2
HEK293
HeLa
HUVEC
K562
L929
MDCK
NIH3T3
P19
PC-12
Vero
Features and Benefits
- Suitable for stable and transient transfection
- High efficiency for a wide variety of cell types
- Compatible with both serum and serum-free transfection protocols
- Low toxicity
Components
Escort™ II Transfection Kit (0.75 mL) contains:
0.75 mL Escort II Reagent (L6037)
0.8 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Escort™ II Transfection Kit (1.5 mL) contains:
1.5 mL Escort II Reagent (L6037)
1.6 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Note the formulation of Escort™ II is confidential.
0.75 mL Escort II Reagent (L6037)
0.8 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Escort™ II Transfection Kit (1.5 mL) contains:
1.5 mL Escort II Reagent (L6037)
1.6 mL Hydration Buffer (B1180)
4 mL DNA Dilutent (D6813)
Note the formulation of Escort™ II is confidential.
Caution
Do not freeze.
Principle
A stable complex is formed when Escort™ II is mixed with DNA in the absence of serum. The complexes are stable and can be directly added to the cell culture medium, where they fuse with the cell membrane, releasing the DNA into the cytoplasm. Note: complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum is without consequence.
Legal Information
Escort is a trademark of Sigma-Aldrich Co. LLC
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Pricing
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
新产品
Certificates of Analysis (COA)
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