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Merck
CN

L3540

Lowry Reagent

Synonym(s):

Copper(II) tartrate Mix

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

Key Documents

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Quality Level

100

Application

For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.
Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

Preparation Note

Reconstitute to 40 mL with deionized water.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

Storage Class

4.1B - Flammable solid hazardous materials

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
0000491218
0000501864
0000501864
0000491218
0000484835

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Environmental and genetic perturbations reveal different networks of metabolic regulation
Greenberg A, et al.
Molecular Systems Biology, 7(1), 563-563 (2011)
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)
Raffinose, a plant galactoside, inhibits Pseudomonas aeruginosa biofilm formation via binding to LecA and decreasing cellular cyclic diguanylate levels
Kim HS, et al.
Scientific reports, 6(1), 25318-25318 (2016)
Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14
Kim HS and Park HD
PLoS ONE, 8(9), e76106-e76106 (2013)
So-Young Ham et al.
Chemosphere, 268, 128802-128802 (2020-11-10)
Biofouling is a major operational problem in the reverse osmosis (RO) process, affecting the membrane performance. Although sodium hypochlorite (NaOCl) is used to chemically clean the biofouled membranes, high concentrations of NaOCl cause morphological and chemical damage to the RO
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Protocols

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

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Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Product Information Sheet - L3540

Instructions

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