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L3540

Supelco

Lowry Reagent

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Synonym(s):
Copper(II) tartrate Mix
UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

100

General description

The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.

Application

For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.
Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.

Other Notes

Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.

Reconstitution

Reconstitute to 40 mL with deionized water.

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Description
Pricing

Signal Word

Danger

Hazard Statements

H228,H302,H314,H412

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)
Environmental and genetic perturbations reveal different networks of metabolic regulation
Greenberg A, et al.
Molecular Systems Biology, 7(1), 563-563 (2011)
Raffinose, a plant galactoside, inhibits Pseudomonas aeruginosa biofilm formation via binding to LecA and decreasing cellular cyclic diguanylate levels
Kim HS, et al.
Scientific reports, 6(1), 25318-25318 (2016)
Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14
Kim HS and Park HD
PLoS ONE, 8(9), e76106-e76106 (2013)
So-Young Ham et al.
Chemosphere, 268, 128802-128802 (2020-11-10)
Biofouling is a major operational problem in the reverse osmosis (RO) process, affecting the membrane performance. Although sodium hypochlorite (NaOCl) is used to chemically clean the biofouled membranes, high concentrations of NaOCl cause morphological and chemical damage to the RO
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Protocols

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Related Content

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

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