G3293
Glucose Assay Reagent
for use with enzymatic assay kits GAHK20, SCA20, FA20, SA20
About This Item
Quality Level
analyte chemical class(es)
sugars (glucose)
technique(s)
photometry: suitable
impurities
≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase
<0.01% ATP-ase, myokinase
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Specificity
Application
related product
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Is it possible to adapt Product G3293, Glucose Assay Reagent, for use with a 96 well plate reader?
In principle, it should be possible to adapt assay procedures using this reagent to 96-well plate readers. Volumes of reagent, water, standard, and unknown should be reduced to fit the capacity of the test wells, and kept in the same proportions as those used with a spectrophotometer. The volume reduction will likely be 1:4 or 1:5. With the shorter "pathlength" of a multiwell plate, the sensitivity of the assay would likely decrease.
Is magnesium required for Product G3293, Glucose Assay Reagent?
Magnesium, an activator of hexokinase, is included in the assay reagent. There is no need to add a magnesium salt to the reagent before use.
What is the detection limit of Product G3293, Glucose Assay Reagent?
This reagent should be able to detect glucose down to approximately 0.01 mg/mL using a spectrophotometer with a 1 cm path length.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols
Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)
To standardize an enzymatic assay procedure of cellulase.
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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