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F7264

Sigma-Aldrich

Fixing solution

5 × (solution)

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Synonym(s):
pre-stain fixing solution
UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

concentration

5 × (solution)

technique(s)

protein staining: suitable

Application

An effective protein fixing solution for pre-stain fixing, suitable for SDS-PAGE, non-denaturing PAGE and IEF. Proteins require fixing into the gel for analytical purposes so that they do not leach out following electrophoresis.

Other Notes

60% (w/v) trichloroacetic acid, 17.5% (w/v) 5-sulfosalicylic acid.

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Protocols

Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

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