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C3041

Sigma-Aldrich

Carbonate-Bicarbonate Buffer

capsule

Synonym(s):

Carbonate Buffer Solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

capsule

Quality Level

pH

9.3-9.9

solubility

water, high purity: 1 capsule/100 mL

application(s)

diagnostic assay manufacturing

Related Categories

General description

Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.

Application

Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay. It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.

Reconstitution

Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.

recommended

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Ashfaq Ghumra et al.
PloS one, 6(1), e16414-e16414 (2011-02-10)
Rosetting is a Plasmodium falciparum virulence factor implicated in the pathogenesis of life-threatening malaria. Rosetting occurs when parasite-derived P. falciparum Erythrocyte Membrane Protein One (PfEMP1) on the surface of infected erythrocytes binds to human receptors on uninfected erythrocytes. PfEMP1 is
Yuki Noguchi et al.
Scientific reports, 10(1), 16268-16268 (2020-10-03)
In vivo pharmacokinetics (PK) studies using mice and monkeys are the main approaches for evaluating and predicting the PK of antibodies, and there is a strong demand for methods that do not require animal experiments. In this work, we focused
Investigation of biofilm formation in clinical isolates of Staphylococcus aureus
Cassat JE, et al.
Methods in Molecular Biology, 391(1), 127-144 (2007)
L von Gersdorff Jørgensen et al.
Journal of fish diseases, 40(12), 1815-1821 (2017-05-27)
The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)

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