Skip to Content
Merck
CN
All Photos(1)

Documents

Safety Information

C-28075

Sigma-Aldrich

3D Tumorsphere Medium XF

Ready-to-use kit including Basal Medium and SupplementMix, phenol red-free, 250 ml

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 250 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

3D Tumosphere Medium XF, phenol red-free

Application

The 3D Tumorsphere Medium XF is a culture system developed for the standardized serial culture of cancer cell lines as tumorspheres/mammospheres. The formulation supports sustained cell proliferation allowing for serial passage of the 3D culture. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials.3D Tumorsphere Medium XF consists of a bottle of phenol red-free Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.

Quality

All lots of the PromoCell 3D Tumorsphere Medium XF are subjected to comprehensive quality control tests using human mammary cancer cells. Each lot of PromoCell 3D Tumorsphere Medium XF is tested for the ability to support tumorsphere/mammosphere formation, proliferation and serial passage of the 3D culture. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

3D Tumorsphere Medium XF contains all growth factors and supplements. The medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.Thaw the SupplementMix at 15 to 25°C. Aseptically mix the supplement solution by carefully pipetting up and down. Then, transfer the entire content of the SupplementMix to the Basal Medium. Close the bottle and swirl gently until a homogenous mixture is formed.

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

WGK

WGK 2

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Protocols

Tumor Spheroid Formation Assay

3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.

In Vitro Differentiation of Human PBMC and CD34+ Derived Monocytes into Mature CD83+/CD14- Dendritic Cells

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

Related Content

3D Mammosphere Culture Media

3D mammosphere culture protocol used to expand human MCF-7 and MDA-MB-231 breast cancer cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service