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A4503

Sigma-Aldrich

Bovine Serum Albumin

cold ethanol fraction, pH 5.2, ≥96%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

300

Assay

≥96%

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

origin

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Application

  • Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
  • It has been used in the cytotoxicity assay buffer.
  • It has been used for the induction of protein overload nephropathy in rats.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Is Product No. A4503, Albumin from bovine serum, tested and found to be protease-free?

    No. If you need such a grade, we would recommend Product No. A3059.

  4. Is Product No. A4503, Albumin from bovine serum, tested and found to be endotoxin-free?

    No. If you need such a grade, we would recommend Product No. A8806.

  5. Is Product No. A4503, Albumin from bovine serum, of US origin?

    It is typically from animals born and raised in the US. You may confirm this information by downloading the Certificate of Origin (CofO) for any lot of A4503 from our website. From the product detail page, simply type in the lot number in the box below the words Certificate of Origin, and click GO.

  6. Is Product No. A4053, Albumin from bovine serum, sterile? If not, how would recommend sterilizing it?

    This product is not necessarily sterile. Solutions of albumin cannot be autoclaved, so in order to sterilize it we would recommend that it be dissolved in the appropriate medium (water, saline or buffered saline), followed by sterile filtration through a low protein-binding membrane.

  7. Do you offer a kit for conjugating ligands to bovine serum albumin (Product A4503)?

    Yes. It is our Product No. MBK1.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sensitization of tumor cells to NK cell-mediated killing by proteasome inhibition.
Hallett WH et al.
Journal of Immunology, 180, 163-163 (2008)
Isabella Marcomini et al.
Cell reports, 24(10), 2614-2628 (2018-09-06)
Multiple pathways regulate the repair of double-strand breaks (DSBs) to suppress potentially dangerous ectopic recombination. Both sequence and chromatin context are thought to influence pathway choice between non-homologous end-joining (NHEJ) and homology-driven recombination. To test the effect of repetitive sequences
Cytochrome C effect on respiration of heart mitochondria: influence of various factors.
Toleikis A et al.
Bioscience Reports, 25, 387-387 (2005)
Simona Ranallo et al.
Nature communications, 8, 15150-15150 (2017-05-10)
A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we
Rapamycin worsens renal function and intratubular cast formation in protein overload nephropathy.
Coombes JD et al.
Kidney International, 68, 2599-2599 (2005)
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Nuclease S1 Enzymatic Assay

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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