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A0847

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein

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About This Item

UNSPSC Code:
41106305
MDL number:
MFCD00675046
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recombinant

expressed in E. coli

Quality Segment

assay

≥70% (SDS-PAGE)

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

177 kDa by amino acid sequence

storage temp.

2-8°C

Gene Information

human ... CAMP(820)
mouse ... CAMP(12796)
rat ... CAMP(316010)

Biochem/physiol Actions

Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.

Preparation Note

When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.

Other Notes

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Storage Class

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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