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75164

Sigma-Aldrich

4-Methylumbelliferyl oleate

lipase substrate, ≥95% (HPCE), solid, suitable for fluorescence

Synonym(s):

Oleic acid 4-methylumbelliferyl ester

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25 MG
¥1,932.63
100 MG
¥5,832.80

¥1,932.63

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25 MG
¥1,932.63
100 MG
¥5,832.80

About This Item

Empirical Formula (Hill Notation):
C28H40O4
CAS Number:
18323-58-5
Molecular Weight:
440.61
Beilstein:
4887028
EC Number:
242-210-7
MDL number:
MFCD00076095
UNSPSC Code:
12352204
PubChem Substance ID:
57652555
NACRES:
NA.21

¥1,932.63

Please contact Customer Service for Availability

Product Name

4-Methylumbelliferyl oleate, suitable for fluorescence, ≥95% (HPCE)

Assay

≥95% (HPCE)

form

solid

storage condition

protect from light
under inert gas

mp

37-38 °C (lit.)

solubility

2-methoxyethanol: soluble
DMSO: soluble
chloroform: soluble

fluorescence

λex 312 nm in methanol
λex 327 nm; λem 449 nm in 0.1 M phosphate pH 7.0 (lipase)

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

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马来酸 Vetec™, reagent grade, 98%

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马来酸 ReagentPlus®, ≥99% (HPLC)

M0375

马来酸

马来酸 ≥99.5% (HPLC), <=0.5% water

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马来酸

马来酸 Standard for quantitative NMR, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

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assay

98%

assay

≥99% (HPLC)

assay

≥99.5% (HPLC), ≥99.5% anhydrous basis (HPLC)

assay

-

form

powder

form

powder

form

powder

form

-

solubility

water: 333 mg/mL, clear, colorless

solubility

-

solubility

-

solubility

-

mp

130-135 °C (lit.)

mp

130-135 °C (lit.)

mp

130-135 °C (lit.), 137-140 °C (lit)

mp

130-135 °C (lit.)

density

1.59 g/mL at 25 °C (lit.)

density

1.59 g/mL at 25 °C (lit.)

density

1.59 g/mL at 20 °C (lit.), 1.59 g/mL at 25 °C (lit.)

density

1.59 g/mL at 25 °C (lit.)

Application

4-Methylumbelliferyl oleate has been used:
  • as a substrate to assay the activity of non-specific esterases[1]
  • to determine lipase activity[2]
  • as a substrate to evaluate pancreatic lipase inhibitory activity[3]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Fluorogenic substrate for lipasecitation
Fluorogenic substrate for lipase[4]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCL3463
    BCCK7519
    BCCH0013
    BCCJ0329
    BCCF8781

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    Lysosomal acid lipase in cultivated fibroblasts: characterization of enzyme activity in normal and enzymatically deficient cell lines.
    B K Burton et al.
    Clinica chimica acta; international journal of clinical chemistry, 101(1), 25-32 (1980-02-14)
    Oleate lipase activity in Gardnerella vaginalis and reconsideration of existing biotype schemes
    Moncla BJ and Pryke KM
    BMC Microbiology, 9(1), 78-78 (2009)
    Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone.
    T J Jacks et al.
    Analytical biochemistry, 21(2), 279-285 (1967-11-01)
    Non-flavonoid phenolics from Averrhoa carambola fresh fruit
    Jia X, et al.
    Journal of functional foods, 32(1), 419-425 (2017)
    T J Van Berkel et al.
    Biochimica et biophysica acta, 617(3), 446-457 (1980-03-21)
    (1) Parenchymal and non-parenchymal cells were isolated from rat liver. The characteristics of acid lipase activity with 4-methylumbelliferyl oleate as substrate and acid cholesteryl esterase activity with cholesteryl[1-14C]oleate as substrate were investigated. The substrates were incorporated in egg yolk lecithin
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