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HLPPMAG-57K

Millipore

MILLIPLEX® Human Liver Protein Magnetic Bead Panel - Metabolism Multiplex Assay

The Human Liver Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 9 liver protein biomarkers in human serum, plasma and tissue/cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 100.2%
(HGF)
accuracy: 93.7%
(ANGPTL3)
accuracy: 94.2%
(AFP)
accuracy: 96.6%
(FGF-23)
accuracy: 96.8%
(ANGPTL4)
accuracy: 98.8%
(FGF-21)
sensitivity: 0.004 ng/mL
(MinDC+2SD; FGF-21)
sensitivity: 0.014 ng/mL
(MinDC+2SD; FGF-23)
sensitivity: 0.018 ng/mL
(MinDC+2SD; HGF)
sensitivity: 0.054 ng/mL
(MinDC+2SD; FGF-19)
sensitivity: 0.144 ng/mL
(MinDC+2SD; AFP)
sensitivity: 0.218 ng/mL
(MinDC+2SD; FABP1)
sensitivity: 0.725 ng/mL
(MinDC+2SD; ANGPTL3)
sensitivity: 0.847 ng/mL
(MinDC+2SD; ANGPTL6)
sensitivity: 2.150 ng/mL
(MinDC+2SD; ANGPTL4)
standard curve range: 0.01-10 ng/mL
(FGF-21)
standard curve range: 0.04-30 ng/mL
(FGF-23)
standard curve range: 0.07-50 ng/mL
(HGF)
standard curve range: 0.14-100 ng/mL
(AFP)
standard curve range: 0.14-100 ng/mL
(FGF-19)
standard curve range: 0.27-200 ng/mL
(FABP1)
standard curve range: 0.69-500 ng/mL
(ANGPTL3)
standard curve range: 1.37-1000 ng/mL
(ANGPTL6/AGF)
standard curve range: 5.49-4000 ng/mL
(ANGPTL4)
inter-assay cv: <20%
intra-assay cv: <10%
(AFP)
inter-assay cv: <20%
intra-assay cv: <10%
(ANGPTL3)
inter-assay cv: <20%
intra-assay cv: <10%
(ANGPTL4)
inter-assay cv: <20%
intra-assay cv: <10%
(ANGPTL6/AGF)
inter-assay cv: <20%
intra-assay cv: <10%
(FABP1)
inter-assay cv: <20%
intra-assay cv: <10%
(FGF-19)
inter-assay cv: <20%
intra-assay cv: <10%
(FGF-21)
inter-assay cv: <20%
intra-assay cv: <10%
(FGF-23)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

Related Categories

General description

Liver-secreted proteins play important roles in metabolic homeostasis through regulation of circulating lipoprotein levels, energy expenditure, glucose metabolism, and fatty acid uptake. Liver-secreted proteins may serve as biomarkers for liver diseases and gastric cancer. Accurate measurement of liver proteins is critical to obtaining an understanding of their biological functions.

MILLIPLEX® Human Liver Protein Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell culture samples: AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1/L-FABP, FGF-19, FGF-21, FGF-23 and HGF.

NOTE: When assaying ANGPTL6/AGF it is recommended that serum samples be used.

This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificity

Cross Reactivty
The ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Application

  • Analytes: α-Fetoprotein (AFP), ANGPTL3, ANGPTL4, ANGPTL6 (AGF), FABP1 (L-FABP), FGF-19, FGF-21, FGF-23, HGF
  • Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
  • NOTE: Serum samples are recommended when measuring ANGPTL6/AGF
  • Recommended Sample Dilution: 12.5 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be used undiluted or appropriately diluted in control medium.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C is recommended for optimal sensitivity. 2 hours at room temperature (20-25° C) may also be used.
  • Research Category: Metabolism
  • Research Subcategory: Metabolic Disorders

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate
Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

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Manjunath Ramanjaneya et al.
Frontiers in endocrinology, 11, 568500-568500 (2020-10-27)
Background: The fibroblast growth factors (FGF) 19 subfamily, also referred to as endocrine FGFs, includes FGF19, FGF21, and FGF23 are metabolic hormones involved in the regulation of glucose and lipid metabolism. Fetuin-A is a hepatokine involved in the regulation of
Nigel Davies et al.
Molecular therapy. Nucleic acids, 24, 369-384 (2021-04-20)
Lipid nanoparticles (LNPs) are the most clinically advanced delivery system for RNA-based drugs but have predominantly been investigated for intravenous and intramuscular administration. Subcutaneous administration opens the possibility of patient self-administration and hence long-term chronic treatment that could enable messenger
Imad Tarhoni et al.
Journal of immunoassay & immunochemistry, 39(1), 84-98 (2018-01-09)
Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. Antigen "capture" antibodies for
Stefano Bartesaghi et al.
Molecular therapy. Nucleic acids, 28, 500-513 (2022-05-21)
Fibroblast growth factor 21 (FGF21) is a promising therapeutic agent for treatment of type 2 diabetes (T2D) and non-alcoholic steatohepatitis (NASH). We show that therapeutic levels of FGF21 were achieved following subcutaneous (s.c.) administration of mRNA encoding human FGF21 proteins.

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service