17-1010
Magna ChIP®-Seq ChIP and NGS Library Preparation Kit
chromatin IP & next generation sequencing library preparation from genomic DNA.
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About This Item
Quality Level
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
sample preparation: suitable
General description
Application
Features and Benefits
- Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin
- Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA
- Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days.
- Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class.
- Enzymes and buffers provided in convenient master mix formulations streamlines library construction
- Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set.
- Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer II
Packaging
- 10 library constructions.
- Three modules containing all key reagents for chromatin immunoprecipitation and next generation sequencing library preparation from genomic DNA.
Preparation Note
Other Notes
- Magna ChIP A/G (2° to 8°C) -Chromatin IP Reagents
- Magna ChIP A/G (-20°C) -Chromatin IP Reagents
- Magna ChIP-Seq Library Construction (-20°C) Reagents
Legal Information
Disclaimer
Storage Class Code
10 - Combustible liquids
Regulatory Information
Certificates of Analysis (COA)
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Related Content
Genome-wide mapping of DNA-protein interactions and epigenetic marks using chromatin immunoprecipitation combined with next generation sequencing (ChIP-Seq) is indispensable for many gene regulation studies. The Magna ChIPSeq Kit simplifies this technique, enabling the performance of ChIP-Seq by virtually any laboratory.
Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.
The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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