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The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
Description
Properties
shipped in:
shipped in:
shipped in:
wet iceshipped in:
manufacturer/tradename:
manufacturer/tradename:
manufacturer/tradename:
Chemicon®manufacturer/tradename:
form:
powderform:
powderform:
powderform:
powderinput:
input:
input:
sample type induced pluripotent stem cell(s)sample type: human embryonic stem cell(s)
input:
storage temp.:
2-8°Cstorage temp.:
2-8°Cstorage temp.:
storage temp.:
2-8°Ctechnique(s):
technique(s):
technique(s):
cell culture | stem cell: suitabletechnique(s):
Quality Level:
200Quality Level:
200Quality Level:
100Quality Level:
200Usage and Safety
Application
Collagenase Type II (Cls II) has been used:
- as a component of Roswell park memorial Institute (RPMI)-1640 media to digest hepatic tissues
- to perfuse murine heart for the isolation of epicardial stromal cells (EpiSC)
- to digest collagenase and inject it into the intrarenal aorta abdominalis to perfuse the kidneys
Collagenase Type IV, Cls IV may be used:
- as a component in Dulbecco′s modified Eagle′s medium (DMEM)/F12 to digest endometrial samples isolated from in vitro fertilization (IVF) patients
- for collagenase perfusion to isolate hepatocytes using collagenase-liver perfusion method in rats
- in the isolation of rat glomerular endothelial cells (GEndCs)
Risk and Safety
Signal Word:
Danger
Hazard Statements: H315,H319,H334,H335
Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
Hazard Classifications:
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs:
Respiratory system
Storage Class Code:
11 - Combustible Solids
WGK:
WGK 1
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
