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Quality Level
form
emulsion
contains
emulsifier (different from those present in Antifoam A emulsion)
technique(s)
cell culture | hybridoma: suitable
microbiological culture: suitable
Related Categories
General description
Antifoam eliminates excessive foaming formed during impingement, due to proteins and/or carbohydrates in the collection medium. Antifoam B emulsion is a silicone based antifoam. Antifoam B emulsion does not affect the growth of bacteria. Antifoam B emulsion functions as an antifoaming agent in shake flask culture of bacteria.
Application
Antifoam B emulsion has been used:
- to enhance collection efficiency in collection media
- as a component in stress granule lysis buffer to reduce foaming, in host cell lysis buffer to reduce foaming caused by detergents
- to improve solubility of antibiotics
- as a foaming controls in bacterial growth media
Other Notes
A 10% aqueous emulsion of polydimethylsiloxane
WGK
WGK 3
Flash Point(F)
>214.0 °F
Flash Point(C)
> 101.1 °C
Certificates of Analysis (COA)
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Fermentation pH modulates the size distributions and functional properties of Gluconobacter albidus TMW 2.1191 levan
Ua-Arak T, et al.
Frontiers in Microbiology, 8, 807-807 (2017)
High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts
Hoffmann S, et al.
Frontiers in Cellular and Infection Microbiology, 8, 43-43 (2018)
Separation of metabolic supply and demand: aerobic glycolysis as a normal physiological response to fluctuating energetic demands in the membrane
Epstein T, et al.
Cancer & Metabolism, 2(1), 7-7 (2014)
Isolation of yeast and mammalian stress granule cores
Wheeler JR, et al.
Methods, 126, 12-17 (2017)
Celeste J Brown et al.
PloS one, 8(3), e60401-e60401 (2013-03-28)
Observing organisms that evolve in response to strong selection over very short time scales allows the determination of the molecular mechanisms underlying adaptation. Although dissecting these molecular mechanisms is expensive and time-consuming, general patterns can be detected from repeated experiments
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