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Safety Information

SM2021

Sigma-Aldrich

MISSION® shRNA Mouse Gene Family Set, DNA

Tyrosine Kinases

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Select a Size

100 G
CN¥911.90
250 G
CN¥1,306.12
500 G
CN¥2,444.50
1 KG
CN¥4,264.48
5 KG
CN¥15,273.54
10 KG
CN¥25,522.00
25 KG
CN¥43,648.17
25 KG
CN¥58,613.10

CN¥911.90

Available to ship onApril 25, 2025Details

Request a Bulk OrderRequest more information
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Select a Size

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100 G
CN¥911.90
250 G
CN¥1,306.12
500 G
CN¥2,444.50
1 KG
CN¥4,264.48
5 KG
CN¥15,273.54
10 KG
CN¥25,522.00
25 KG
CN¥43,648.17
25 KG
CN¥58,613.10

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

CN¥911.90

Available to ship onApril 25, 2025Details

Request a Bulk OrderRequest more information

Quality Level

200

product line

MISSION®

storage temp.

−20°C

Gene Information

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1 of 4

This Item
T5941T15760T6666
Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline

T3253

Trizma® hydrochloride

Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

T5941

Trizma® hydrochloride

Trizma® hydrochloride reagent grade, ≥99%, crystalline

T15760

Trizma® hydrochloride

Trizma® hydrochloride BioXtra, pH 3.5-5.0 (0.5 M in H2O), ≥99.0% (titration)

T6666

Trizma® hydrochloride

technique(s)

affinity chromatography: suitable

technique(s)

DNA purification: suitable, cell culture | mammalian: suitable, electrophoresis: suitable, protein extraction: suitable

technique(s)

-

technique(s)

-

assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99%

assay

≥99.0% (titration)

grade

reagent grade

grade

BioPerformance Certified

grade

reagent grade

grade

-

Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

400

application(s)

diagnostic assay manufacturing
life science and biopharma
sample preparation

application(s)

diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation

application(s)

-

application(s)

diagnostic assay manufacturing

form

crystalline

form

crystalline powder

form

crystalline

form

powder

General description

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Number of Genes: 133, Number of Cones: 850
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Cecilia Annerén
    Clinical science (London, England : 1979), 115(2), 43-55 (2008-06-13)
    Pluripotent ES (embryonic stem) cells can be expanded in culture and induced to differentiate into a wide range of cell types. Self-renewal of ES cells involves proliferation with concomitant suppression of differentiation. Some critical and conserved pathways regulating self-renewal in
    Paolo M Comoglio et al.
    Nature reviews. Drug discovery, 7(6), 504-516 (2008-05-31)
    The MET tyrosine kinase stimulates cell scattering, invasion, protection from apoptosis and angiogenesis, thereby acting as a powerful expedient for cancer dissemination. MET can also be genetically selected for the long-term maintenance of the primary transformed phenotype, and some tumours

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