SHC216
MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA
Targets no known genes from any species
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Synonym(s):
MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
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Quality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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General description
The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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Regulatory Information
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