SHC002
MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA
Targets no known mammalian genes
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Synonym(s):
MISSION® Control Vectors
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Quality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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Application
MISSION® pLKO.1-puro non-mammalian shRNA control plasmid DNA has been used:
- to transduce RLE-6TN cells
- for the validation of Oct4-green fluorescent protein (GFP) mouse embryonic fibroblasts (MEFs)
- as a negative control for RNA knockdown
MISSION®pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA has been used:
- for the knockdown of transmembrane prostate androgen-induced RNA (TMEPAI)
- in the infection mixture for lentivirus preparation
- for generating the puromycin-resistant ST14A (rat striatal neuronal cell line) cell populations
- to induce Ago2 (argonaute-2) down-regulation
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
recommended
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Platelet-independent adhesion of calcium-loaded erythrocytes to von Willebrand factor.
Smeets MW
PLoS ONE, 12.3 (2017)
TRIM28 is an Epigenetic Barrier to Induced Pluripotent Stem Cell Reprogramming.
Miles DC
Stem Cells (2017)
Identification of a gene expression signature associated with the metastasis suppressor function of NME1: prognostic value in human melanoma
Leonard MK, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 98(3), 327-327 (2018)
TMEPAI/PMEPA 1 enhances tumorigenic activities in lung cancer cells
Vo Nguyen TT, et al.
Cancer Science, 105(3), 334-341 (2014)
The metastasis suppressor NME1 inhibits melanoma cell motility via direct transcriptional induction of the integrin beta-3 gene
Leonard MK, et al.
Experimental Cell Research, 374(1), 85-93 (2019)
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