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SHC001

Sigma-Aldrich

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA

Contains no shRNA insert

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Synonym(s):
MISSION® Control Vectors
MDL number:
MFCD07785395
UNSPSC Code:
12352200
NACRES:
NA.51

Quality Level

200

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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General description

The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The pLKO.1-puro Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA has been used to synthesize and clone shRNAs (which target murine cluster of differentiation 36 (CD36), to generate lentiviral expression vectors.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Where is the hairpin inserted with Product SHC001, MISSION® pLKO.1-puro Control Vector?

    This control vector does not contain an shRNA insert.

  4. What is the function of this negative control with Product SHC001, MISSION® pLKO.1-puro Control Vector?

    It permits observation of cellular effects resulting from the transfection process. Cells transfected with the empty control provide a useful reference point for determining specific knockdown.

  5. Does Product SHC001, MISSION® pLKO.1-puro Control Vector, convey puromycin resistance to transfected cells?

    Yes, it has the puromycin resistance gene.

  6. Is Product SHC001, MISSION® pLKO.1-puro Control Vector, available as a glycerol stock?

    The control vectors are available in plasmid DNA or the lentiviral transduction format.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Cardiospecific CD36 suppression by lentivirus-mediated RNA interference prevents cardiac hypertrophy and systolic dysfunction in high-fat-diet induced obese mice.
Zhang Y
Cardiovascular Diabetology, 14 (2015)
AMP-activated protein kinase (AMPK)-induced preconditioning in primary cortical neurons involves activation of MCL-1.
Anilkumar U
Journal of Neurochemistry, 124 (2013)
Gregory J Baker et al.
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Irene Rodríguez-Sánchez et al.
PLoS pathogens, 15(1), e1007569-e1007569 (2019-01-25)
Human Cytomegalovirus (HCMV) infection induces several metabolic activities that are essential for viral replication. Despite the important role that this metabolic modulation plays during infection, the viral mechanisms involved are largely unclear. We find that the HCMV UL38 protein is
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shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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