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Safety Information

SH2931

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Lentiviral Particles

B-Cell Activation

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1 KIT
CN¥5,282.92

CN¥5,282.92

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1 KIT
CN¥5,282.92

About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

CN¥5,282.92

Please contact Customer Service for Availability
New, lower price on this item!
Request a Bulk OrderRequest more information

Quality Level

200

product line

MISSION®

storage temp.

−70°C

Gene Information

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小鼠 TIMP-1 ELISA 试剂盒 for serum, plasma and cell culture supernatant

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小鼠 TIMP-1 ELISA 试剂盒 for cell and tissue lysates

RAB0469

小鼠 TIMP-1 ELISA 试剂盒

小鼠 TIMP-2 ELISA 试剂盒 for serum, plasma and cell culture supernatant

RAB0473

小鼠 TIMP-2 ELISA 试剂盒

人类 TIMP-1 ELISA 试剂盒 for cell and tissue lysates

RAB0467

人类 TIMP-1 ELISA 试剂盒

Gene Information

mouse ... Timp1(21857)

Gene Information

mouse ... Timp1(21857)

Gene Information

mouse ... Timp2(21858)

Gene Information

human ... TIMP1(7076)

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

human

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 1.37-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 1.37-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.0823-60 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 24.69-18000 pg/mL

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type cell lysate
sample type tissue lysate

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type tissue lysate
sample type cell lysate

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Aliquots of 4 x 50μl are provided to prevent the need for excessive freeze/thaw, and maximize functional viral particles. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 99, Number of Clones: 661
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Pavel Tolar et al.
    Immunological reviews, 221, 64-76 (2008-02-16)
    The binding of antigen to the B-cell receptor (BCR) induces BCR clustering and signaling cascades that lead to the activation of a variety of genes associated with B-cell activation. Over the last several years, our understanding of the molecular details
    Pierre Youinou
    Journal of autoimmunity, 28(2-3), 143-151 (2007-03-17)
    The interest for B cells has recently been revived. They normally play a role in the development, the regulation, as well as the activation of lymphoid architecture: they regulate dendritic cells and T-cell subsets function through cytokine production. Receptor editing
    Joshua P Klopper et al.
    Molecular cancer, 8, 16-16 (2009-03-10)
    Metastatic melanoma has a high mortality rate and suboptimal therapeutic options. Molecular targeting may be beneficial using the rexinoid LGD1069, a retinoid x receptor selective agonist, and thiazolidinediones (TZD), PPARgamma selective ligands, as novel treatments. Mouse xenograft models with human
    Courtney G Woods et al.
    Toxicology and applied pharmacology, 238(1), 27-36 (2009-04-21)
    Hypochlorous acid (HOCl) is potentially an important source of cellular oxidative stress. Human HOCl exposure can occur from chlorine gas inhalation or from endogenous sources of HOCl, such as respiratory burst by phagocytes. Transcription factor Nrf2 is a key regulator

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