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Key Documents

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Safety Information

R7023

Sigma-Aldrich

Pst I from Providencia stuartii

Restriction Enzyme

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1 G
CN¥1,464.20

CN¥1,464.20

Available to ship onApril 23, 2025Details

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1 G
CN¥1,464.20

About This Item

CAS Number:
81295-32-1
Enzyme Commission number:
3.1.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00165594
UNSPSC Code:
12352204

CN¥1,464.20

Available to ship onApril 23, 2025Details

grade

Molecular Biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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1 of 4

This Item
404308552047848
Potassium sorbate Pharmaceutical Secondary Standard; Certified Reference Material

PHR1278

Potassium sorbate

Potassium sorbate certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

40430

Potassium sorbate

Potassium sorbate purum p.a., ≥99.0% (NT)

85520

Potassium sorbate

Potassium Sorbate analytical standard

47848

Potassium Sorbate

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

100

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, TraceCERT®

grade

-

grade

analytical standard

storage temp.

2-30°C

storage temp.

-

storage temp.

-

storage temp.

2-30°C

agency

traceable to Ph. Eur. P2650000

agency

-

agency

-

agency

-

Specificity

Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Application

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Pst I requires optimal reaction conditions in order to avoid star activity.

Physical form

Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    Certificates of Analysis (COA)

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    SLBN0525V
    SLBL8959V
    SLBK8116V
    SLBH5596V
    SLBH5596

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    T B Britschgi et al.
    Applied and environmental microbiology, 57(6), 1707-1713 (1991-06-01)
    The identification of the prokaryotic species which constitute marine bacterioplankton communities has been a long-standing problem in marine microbiology. To address this question, we used the polymerase chain reaction to construct and analyze a library of 51 small-subunit (16S) rRNA
    Thomas Hiesberger et al.
    The Journal of clinical investigation, 113(6), 814-825 (2004-04-07)
    Hepatocyte nuclear factor-1beta (HNF-1beta) is a Pit-1, Oct-1/2, UNC-86 (POU)/homeodomain-containing transcription factor that regulates tissue-specific gene expression in the liver, kidney, and other organs. Humans with autosomal dominant mutations of HNF-1beta develop maturity-onset diabetes of the young type 5 (MODY5)
    E Müller et al.
    Pflugers Archiv : European journal of physiology, 435(5), 705-712 (1998-04-16)
    The influence of diuresis and antidiuresis on the expression of heat shock proteins (HSP) 25, 60, 72 and 73 in the renal cortex and outer and inner medulla of Wistar rats was analysed. Medullary osmolality was reduced by long-term diuresis
    The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.
    N L Brown et al.
    FEBS letters, 65(3), 284-287 (1976-06-15)
    D I Smith et al.
    Nucleic acids research, 3(2), 343-353 (1976-02-01)
    We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and
    View All Related Papers

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