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QPBCA

Sigma-Aldrich

QuantiPro™ BCA Assay Kit

for 0.5-30 μg/ml protein

Synonym(s):

BCA Assay, Protein determination by BCA, bicinchoninic acid reagent

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Application

In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Features and Benefits

  • Accurate across a broad range of protein concentrations
  • High sensitivity; linear response form 0.5 to 30 μg/ml of protein
  • Stable color complex
  • Reduced susceptibility to detergents

Principle

Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.

Storage and Stability

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Legal Information

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS

Application

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H290,H315,H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Information

含少量动物源组分生物产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sook Hyun Chung et al.
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Vascular changes and photoreceptor degeneration are features of age-related macular degeneration, diabetic retinopathy and macular telangiectasis. We have profiled the differential expression of microRNAs and analysed their target genes in transgenic mice in which induced Müller cell disruption results in
Eneida V Reis et al.
Parasitology international, 63(1), 120-126 (2013-10-24)
Vaults are ribonucleoproteins (13 MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The
Comparison of the glycopattern alterations of mitochondrial proteins in cerebral cortex between rat Alzheimer?s disease and the cerebral ischemia model
Yu H,et al.
Scientific reports (2017)
PPAR-? agonist GL516 reduces oxidative stress and apoptosis occurrence in a rat astrocyte cell line
Neurochemistry International (2019)
Jingwei Zhang et al.
Acta biomaterialia, 10(7), 3254-3263 (2014-04-01)
The microporosity of calcium phosphate (CaP) ceramics has been shown to have an essential role in osteoinduction by CaP ceramics after ectopic implantation. Here we show that it is not the microporosity but the size of surface microstructural features that
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