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Safety Information

OGS412

Sigma-Aldrich

PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID

plasmid vector for molecular cloning

Synonym(s):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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250 MG
CN¥2,452.68

CN¥2,452.68

Estimated to ship onApril 27, 2025Details

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250 MG
CN¥2,452.68

About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

CN¥2,452.68

Estimated to ship onApril 27, 2025Details

form

buffered aqueous solution

mol wt

size 5475 bp

bacteria selection

kanamycin

Origin of replication

pUC (500 copies)

Peptide cleavage

no cleavage

Promoter

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

reporter gene

firefly luciferase

shipped in

ambient

storage temp.

−20°C

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三磷酸鸟苷 >90% (HPLC), crystals, pkg of 250 mg

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三磷酸鸟苷

鸟苷 5′-三磷酸盐 三羟甲基氨基甲烷盐 ≥93% (HPLC), powder

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鸟苷 5′-三磷酸盐 三羟甲基氨基甲烷盐

鸟苷5′-三磷酸 钠盐 水合物 ≥95% (HPLC), powder

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鸟苷5′-三磷酸 钠盐 水合物

鸟苷5′-三磷酸 钠盐 溶液 HPLC purified, aqueous solution for RNA polymerase transcription

G3776

鸟苷5′-三磷酸 钠盐 溶液

assay

>90% (HPLC)

assay

≥93% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

form

crystals

form

powder

form

powder

form

liquid

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

mol wt

Mr 523.2 (GTP), Mr 567.1 (GTP-Na2)

mol wt

-

mol wt

-

mol wt

-

storage condition

avoid repeated freeze/thaw cycles

storage condition

-

storage condition

-

storage condition

-

General description

The expression of the Firefly luciferase (FLuc Photinus pyralis) reporter gene under the control of the RecA constitutive bacterial promoter. This plasmid can be used for monitoring reporter gene activity in bacterial cells or colonies using the luciferase substrate luciferin.

Promoter Expression Level: PSF-OXB20-FLUC - BACTERIAL LUCIFERASE plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Application

Cloning in a gene: PSF-OXB20-FLUC - BACTERIAL LUCIFERASE plasmid contains a gene within the main multiple cloning site (NotI-ClaI). Any plasmid that we sell where the gene is this configuration will be located in the exact same position in relation to the start and stop codon of the gene. The only exceptions to this rule are fusions proteins where the fusion gene may be positioned at the front or end of the MCS to allow gene fusion.

By positioning all of our genes in the same location it allows them to be transferred between plasmids using the same cloning method and restriction sites regardless of the plasmid being used from our product range. Inserting a new gene into this plasmid should be easily possible using a range of standard restriction enzyme sites that flank the gene currently in the vector.

Multiple cloning site notes: In the multiple cloning site there are two important restriction sites called BsgI and BseRI sites. These sites both cut the DNA at the same position and cleave the stop codon of the gene in the multiple cloning site in this plasmid thereby producing a TA overhang. This overhang is compatible with any of our peptide or reporter fusion tag plasmids also cut with either of these enzymes. This allows seamless C-terminal fusions to be made with the gene in this multiple cloning site using a single cloning step from our C-terminal peptide and reporter tag product range. Normally the easiest method is to clone the C-terminal tag from our other plasmid products into this plasmid using BsgI or BseRI and the downstream ClaI restriction site.

BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding sites. This allows them to cut the upstream stop codon in the gene in this plasmid regardless of the gene sequence.
PSF-OXB20-FLUC -bacterial Luciferase plasmid has been used as a reporter plasmid for Clostridium difficile genes for expression studies.[1] It has also been used in reporter mRNA construct preparations.[2]

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    John P Hegarty et al.
    International journal of nanomedicine, 11, 3607-3619 (2016-08-19)
    Despite being a conceptually appealing alternative to conventional antibiotics, a major challenge toward the successful implementation of antisense treatments for bacterial infections is the development of efficient oligonucleotide delivery systems. Cationic vesicles (bolasomes) composed of dequalinium chloride ("DQAsomes") have been
    Marianna Penzo et al.
    Nature protocols, 11(7), 1309-1325 (2016-06-24)
    We describe a cell-free translation system for evaluating the activity of ribosomes stringently purified from human cells. This system is based on in vitro reconstitution of the cellular translation machinery, in which a ribosome-free rabbit reticulocyte lysate (RRL) is reassembled
    Alexander C Cerny et al.
    PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
    Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
    Geoffrey M Lynn et al.
    Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
    The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
    Diana Romero et al.
    Carcinogenesis, 37(1), 18-29 (2015-10-28)
    Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
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