OGS1157
PSF-CMV-PURO-NH2-10HIS-EKT - N-TERMINAL 10HIS TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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CN¥3,913.36
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About This Item
CN¥3,913.36
tag
10-His tagged
form
buffered aqueous solution
mol wt
size 6194 bp
bacteria selection
kanamycin
mammalian cells selection
puromycin
Origin of replication
pUC (500 copies)
Peptide cleavage
EKT
Peptide tag location
N-terminal
Promoter
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
reporter gene
none
1 of 4
This Item | OGS1158 | OGS1119 | OGS1080 |
|---|---|---|---|
| promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV |
| peptide cleavage EKT | peptide cleavage EKT | peptide cleavage TEV | peptide cleavage thrombin |
| origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) |
| bacteria selection kanamycin | bacteria selection kanamycin | bacteria selection kanamycin | bacteria selection kanamycin |
| mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin |
General description
About the Peptide Tag:This plasmid contains an n-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.
About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Sequence
Analysis Note
related product
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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