M2909
Murashige and Skoog Modified Basal Salt Mixture
powder, suitable for plant cell culture
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Synonym(s):
Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate
UNSPSC Code:
12352207
NACRES:
NA.72
Recommended Products
form
powder
Quality Level
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
Application
Murashige and Skoog Modified Basal Salt Mixture has been used:
- to promote root development for internode stem suckers
- to promote shoot growth for the calluses
- for low nitrogen (LN) treatment, for the growth of Arabidopsis thaliana
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Formula variant
Without NH4NO3
With the macro- and micronutrients described by Murashige and Skoog (1962).
Media Formulation
With the macro- and micronutrients described by Murashige and Skoog (1962).
Media Formulation
Preparation Note
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
易制爆化学品
危险化学品
Certificates of Analysis (COA)
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Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes
Zale JM, et al.
Plant Cell, Tissue and Organ Culture, 76(3), 277-281 (2004)
Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence
Pourtau N, et al.
Planta, 219(5), 765-772 (2004)
Iron homeostasis alteration in transgenic tobacco overexpressing ferritin
Van WO, et al.
The Plant Journal, 17(1), 93-97 (1999)
O Van Wuytswinkel et al.
The Plant journal : for cell and molecular biology, 17(1), 93-97 (1999-03-09)
Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the
Ca2+ regulates reactive oxygen species production and pH during mechanosensing in Arabidopsis roots.
Gabriele B Monshausen et al.
The Plant cell, 21(8), 2341-2356 (2009-08-06)
Mechanical stimulation of plants triggers a cytoplasmic Ca(2+) increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient
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