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H2779

Sigma-Aldrich

HistoChoice® Clearing Agent

greener alternative

alternative to toluene and xylene

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Synonym(s):
dewaxing mixture for paraffin tissue sections
UNSPSC Code:
12352200
NACRES:
NA.47

Quality Level

form

liquid

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

color

colorless

density

0.760-0.820 g/mL

application(s)

hematology
histology

greener alternative category

storage temp.

room temp

General description

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites, and nucleic acid sites. It is a non-toxic, non-cross-linking fixative formulated to replace formaldehyde-based fixation. This formulation in non-citrus, contains no mercury, formaldehyde, or glutaraldehyde.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of formalin for tissue processing.

Application

HistoChoice® Clearing Agent has the following applications:
  • It is suitable as an alternative to toluene and xylene for dewaxing paraffin tissue sections. Prepared slides are suitable for antibody probing applications and in situ hybridizations.
  • It has been employed in a study to explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation.
  • It has been used to develop a novel method to improve sensitivity for the localization of miRNA and their target transcripts in plants.
  • It has been used to study the correlation of cytoplasmic ADP-ribosylation levels with markers of patient outcome in human cancers.

Biochem/physiol Actions

Fixation with HistoChoice® works well in immunohistology applications where its non-cross-linking traits are conducive to epitope retrieval and identification by primary antibodies to extracellular matrix components.

Features and Benefits

  • Non-toxic, non-carcinogenic, and odorless.
  • Non-flammable.
  • Preserves native antigenic and nucleic acid sites.
  • Compatible with many organic mounting media.
  • Dissolves wax faster and evaporates slower than xylene.
  • Can be used in automated processors.
  • Tissues retain their original structure.

Legal Information

HistoChoice is a registered trademark of Amresco, Inc.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

142.0 °F

Flash Point(C)

61.1 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is HistoChoice Clearing Agent, product H2779, compatible with polycarbonate?

    The supplier of HistoChoice Clearing Agent has reported no incompatibilities were observed when polycarbonate was exposed to HistoChoice Clearning Agent for at least 1 hour.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Cytoplasmic ADP-ribosylation levels correlate with markers of patient outcome in distinct human cancers
Aimi, et al.
Modern Pathology (2021)
Marie-Françoise Devaux et al.
Frontiers in plant science, 9, 200-200 (2018-03-09)
Tracking enzyme localization and following the local biochemical modification of the substrate should help explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation. Time-lapse studies using conventional imaging require enzyme labeling and following the biochemical modifications of biopolymers
Anita S Patel et al.
The Journal of investigative dermatology, 128(12), 2888-2893 (2008-06-13)
An increasing number of studies report that genus beta human papillomaviruses (HPVs) are associated with skin cancer, with suggestions of specificity for squamous cell carcinoma (SCC) of the skin. We have conducted a systematic examination of HPV DNA in tumors
Janine A Duckworth et al.
Reproduction (Cambridge, England), 133(1), 177-186 (2007-01-25)
In a previous study, three infertility-relevant epitopes of possum ZP2 (Pep12 (amino acids 111-125), Pep31 (amino acids 301-315), and Pep44 (amino acids 431-445)) were identified using sera from possums (Trichosurus vulpecula) immunized with recombinant possum zona pellucida 2 (ZP2) constructs
Alexander Kunz et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(7), 1649-1658 (2008-02-15)
CD36, a class-B scavenger receptor involved in multiple functions, including inflammatory signaling, may also contribute to ischemic brain injury through yet unidentified mechanisms. We investigated whether CD36 participates in the molecular events underlying the inflammatory reaction that accompanies cerebral ischemia

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