E6783
pFLAG-CMV-3 Expression Vector
shuttle vector for transient or stable expression of N-terminal FLAG
tag
FLAG® tagged
grade
Molecular Biology
form
buffered aqueous solution
peptide tag location
N-terminal
shipped in
dry ice
storage temp.
−20°C
General description
The pFLAG-CMV™-3 Expression Vector is a 6.3 kb vector used for transient or stable expression in
mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.
The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The FLAG® epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.
Vector Maps and Sequences
mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.
The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The FLAG® epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.
Vector Maps and Sequences
Application
pFLAG-CMV™-3 Expression Vector is suitable for transient or stable expression and secretion of N-terminal FLAG-tagged fusion proteins in mammalian cells.
Biochem/physiol Actions
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. The preprotrypsin leader sequence precedes the FLAG® sequence and directs secretion of the fusion protein into the culture medium. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418, allowing for selection of stable transfectants.
Other Notes
- pFLAG-CMV™-3 Expression Vector 20 μg (E8770) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CMV™-3-BAP Control Plasmid 20 μg (C3972) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
Storage Class
10 - Combustible liquids
Regulatory Information
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Instructions
pFLAG-CMV-3 and pFLAG-CMV-4 Vector Maps
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