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Safety Information

C0615

Sigma-Aldrich

Cellulase from Trichoderma sp.

greener alternative

powder, ≥5,000 units/g solid

Synonym(s):

Onozuka RS

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About This Item

CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

Quality Level

200

specific activity

≥5,000 units/g solid

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

solubility

deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))

greener alternative category

Enabling,

storage temp.

2-8°C

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General description

Manufactured by Yakult
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties. Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose while investigating the biodegradability of bioabsorbable bacterial cellulose (BBC).

Biochem/physiol Actions

Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences .

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

Preparation Note

Dissolves in sterile deionized (DI) water in the presence of 0.15 polyhexamethylene biguanide (PHMB) at 3 mg/mL concentration.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Does product C0615, cellulase from Trichoderma viride, contain any sugar as a stabilizer?

    No. Product C0615, cellulase from Trichoderma viride, does not contain any sugar stabilizer.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Youwen Pan et al.
Applied and environmental microbiology, 76(5), 1433-1441 (2010-01-06)
Biofilm formation by Listeria monocytogenes is generally associated with its persistence in the food-processing environment. Serotype 1/2a strains make up more than 50% of the total isolates recovered from food and the environment, while serotype 4b strains are most often
Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
Rey Allen et al.
Applied and environmental microbiology, 85(7) (2019-02-03)
Phototrophic biofilms are key to nutrient cycling in natural environments and bioremediation technologies, but few studies describe biofilm formation by pure (axenic) cultures of a phototrophic microbe. The cyanobacterium Synechocystis sp. strain PCC 6803 (here Synechocystis) is a model microorganism
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Ninian Prem Prashanth Pabbathi et al.
Biomass conversion and biorefinery, 1-28 (2021-01-14)
As the fossil fuel reserves are depleting rapidly, there is a need for alternate fuels to meet the day to day mounting energy demands. As fossil fuel started depleting, a quest for alternate forms of fuel was initiated and biofuel
View All Related Papers

Protocols

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

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