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B6404

Supelco

BCIP®/NBT solution, premixed

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1 KG
CN¥1,462.57
5 KG
CN¥1,974.22

CN¥1,462.57

Available to ship onApril 07, 2025Details

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1 KG
CN¥1,462.57
5 KG
CN¥1,974.22

About This Item

UNSPSC Code:
41105333
NACRES:
NA.32

CN¥1,462.57

Available to ship onApril 07, 2025Details

Request a Bulk Order

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This Item
P5147P6236P6911
Aminopeptidase I from Streptomyces griseus lyophilized powder, ≥200 units/mg protein

A9934

Aminopeptidase I from Streptomyces griseus

Protease from Streptomyces griseus Type XIV, ≥3.5 units/mg solid, powder

P5147

Protease from Streptomyces griseus

Pyroglutamate Aminopeptidase from Pyrococcus furiosus recombinant, expressed in E. coli, ~90% (SDS-PAGE), ≥5.0 units/mg protein

P6236

Pyroglutamate Aminopeptidase from Pyrococcus furiosus

Protease from Streptomyces griseus BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

P6911

Protease from Streptomyces griseus

form

lyophilized powder

form

powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

21 kDa by gel filtration, 33 kDa by SDS-PAGE

mol wt

-

mol wt

24.072 kDa by amino acid sequence, 28 kDa by SDS-PAGE

mol wt

monomer ~20 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, 40-60% Lowry

composition

-

composition

-

composition

-

Application

BCIP/NBT solution, premixed has been used:
  • as a substrate to develop dot-blots[1]
  • as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis[2]
  • to detect specific immune complexes in western blot analysis[3][4][5]

Other Notes

0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2

Substrates

Substrate for visualizing alkaline phosphatase conjugates in Western blotting.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000415346
    0000390474
    0000390474
    0000415346
    0000343857

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    Find documentation for the products that you have recently purchased in the Document Library.

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    The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
    Alcocer M J C, et al.
    Journal of Molecular Biology, 343(3), 759-769 (2004)
    Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
    Tsai R K, et al.
    Experimental Eye Research, 87(3), 242-250 (2008)
    Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
    Tsai R K, et al.
    Experimental Eye Research, 90(5), 537-545 (2010)
    Sandra Fernandes Arruda et al.
    Nutrients, 12(5) (2020-05-28)
    The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
    Slow-and fast-twitch rat hind limb skeletal muscle phenotypes 8 months after spinal cord transection and olfactory ensheathing glia transplantation
    Negredo P, et al.
    The Journal of Physiology, 586(10), 2593-2610 (2008)
    View All Related Papers

    Articles

    Colorimetric Phosphatase/Peroxidase Substrate Systems

    NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service