B6404
BCIP®/NBT solution, premixed
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About This Item
UNSPSC Code:
41105333
NACRES:
NA.32
form
liquid
Quality Level
General description
Substrate for visualizing alkaline phosphatase conjugates in Western blotting.
Application
BCIP/NBT solution, premixed has been used:
- as a substrate to develop dot-blots
- as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
- to detect specific immune complexes in western blot analysis
Other Notes
0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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CD34 is a transmembrane phosphoglycoprotein used to selectively enrich bone marrow in hematopoietic stem cells for transplantation. Treating rats with CD34
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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