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B1911

Sigma-Aldrich

BCIP®/NBT

alkaline phosphatase substrate, chromogenic, liquid

Synonym(s):

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution

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100 ML
CN¥1,085.50

CN¥1,085.50

Available to ship onApril 14, 2025Details

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100 ML
CN¥1,085.50

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

CN¥1,085.50

Available to ship onApril 14, 2025Details

New, lower price on this item!

Product Name

BCIP®/NBT Liquid Substrate System, ready to use solution

form

liquid

storage temp.

2-8°C

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BCIP®/NBT Liquid Substrate System ready to use solution

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BCIP®/NBT-Purple Liquid Substrate System for Membranes ready to use alkaline phosphatase substrate

B3679

BCIP®/NBT-Purple Liquid Substrate System for Membranes

BCIP®/NBT-Blue Liquid Substrate System for Membranes alkaline phosphatase substrate

B3804

BCIP®/NBT-Blue Liquid Substrate System for Membranes

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
  • for staining alkaline phosphatase(ALP) in bone marrow stromal cells[1]
  • as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody[2]
  • as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays[3]

Caution

It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000390393
    0000390393
    0000390399
    0000390399
    0000380391

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    Jungho Jang et al.
    Scientific reports, 8(1), 7211-7211 (2018-05-10)
    The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified
    Salma E Jiménez-Badillo et al.
    Scientific reports, 7(1), 13351-13351 (2017-10-19)
    The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either
    Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
    Ling M, et al.
    Cell & Bioscience, 7(1), 27-27 (2017)
    The evaluation of angiogenesis and matrix metalloproteinase-2 secretion in bone marrow of multiple myeloma patients before and after the treatment
    Bolkun L, et al.
    Advances in Medical Sciences, 58(1), 118-125 (2013)
    Gerry Nganou et al.
    Frontiers in molecular neuroscience, 11, 234-234 (2018-07-26)
    The building of the brain is a multistep process that requires the coordinate expression of thousands of genes and an intense nucleocytoplasmic transport of RNA and proteins. This transport is mediated by karyopherins that comprise importins and exportins. Here, we
    View All Related Papers

    Articles

    Colorimetric Phosphatase/Peroxidase Substrate Systems

    NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

    Protocols

    Immunodetection Using BCIP/NBT Substrate

    BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service