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A4551

Sigma-Aldrich

α-Amylase from Bacillus licheniformis

greener alternative

lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE)

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Synonym(s):
1,4-α-D-Glucan-glucanohydrolase
CAS Number:
9000-85-5
Enzyme Commission number:
3.2.1.1 (BRENDA, IUBMB)
EC Number:
232-560-9
MDL number:
MFCD00081319
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Bacillus licheniformis

Quality Level

200

Assay

93-100% (SDS-PAGE)

form

lyophilized powder

specific activity

500-1,500 units/mg protein

mol wt

58-62 kDa

composition

Protein, 60-80% BCA

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

, Enabling

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Lyophilized powder containing potassium phosphate

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

WGK

WGK 3

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What information is available regarding the solubility of Product A4551, alpha-Amylase from Bacillus licheniformis?

    Product A4551 is a lyophilized alpha-amylase. Although it is reasonable to expect it to dissolve in water or dilute buffer, we do not have a solubility specification; see current specifications on our website. When we measure the specific activity of this enzyme, we typically make our first dilution in cold purified (deionized) water at about 2-3 mg/mL, followed by an intermediate dilution into purified water, followed by dilution into the reaction buffer to start the reaction.When making a solution of the enzyme, it is OK to stir gently with a magnetic stir bar; do not vortex or sonicate.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Douglas W Lowman et al.
Cell surface (Amsterdam, Netherlands), 7, 100061-100061 (2021-11-13)
The fungal cell wall serves as the interface between the organism and its environment. Complex carbohydrates are a major component of the Candida albicans cell wall, i.e., glucan, mannan and chitin. β-Glucan is a pathogen associated molecular pattern (PAMP) composed
Imogen Ramsey et al.
Scientific reports, 10(1), 20855-20855 (2020-12-02)
Consumer sensory evaluation, aroma release analysis and biophysical protein analysis were used to investigate the effect of ethanol on the release and perception of flavour in beer (lager and stout) at different ethanol levels (0 and 5% ABV). Consumer study
Uri Lesmes et al.
Journal of agricultural and food chemistry, 56(13), 5415-5421 (2008-06-12)
This study probed the possible effects of type III resistant starch (RS) crystalline polymorphism on RS fermentability by human gut microbiota and the short chain fatty acids production in vitro. Human fecal pH-controlled batch cultures showed RS induces an ecological
Olivier Etienne et al.
Biomacromolecules, 6(2), 726-733 (2005-03-15)
Biomedical devices and modified biomaterial surfaces constitute an expanding research domain in the dental field. However, such oral applications have to face a very particular environment containing specific physiological conditions and specific enzymes. To evaluate their suitability in the development
Barman, T.E.
Enzyme Handbook, 2, 560-560 (1969)
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Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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