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Merck
CN

A3653

Actin from bovine muscle

lyophilized powder

Synonym(s):

G-Actin

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About This Item

CAS Number:
51005-14-2
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00130338

Key Documents

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Product Name

Actin from bovine muscle, lyophilized powder

biological source

bovine muscle

assay

≥90% (SDS-GE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

western blot: suitable

UniProt accession no.

P62739

storage temp.

2-8°C

Quality Level

200

Gene Information

bovine ... LOC(528168)

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Application

Actin from bovine muscle has been used as an internal standard and positive control during western blotting.

Biochem/physiol Actions

Actin is involved in a wide variety of cellular processes like cell migration, phagocytosis, cell polarity and shape. It associates with myosin and aids in muscle contraction. The protein acts as a building block of microfilaments.

General description

Actin is a highly abundant and conserved protein. It can transition between the monomeric (G-actin) and filamentous (F-actin) forms, which is influenced by external factors. Actin is expressed in smooth, skeletal and cardiac muscles.

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBX5919
SLCB8061
SLBQ7282V
SLBM5951V
SLBD7277V

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T V L Berghof et al.
Poultry science, 89(2), 227-235 (2010-01-16)
Natural antibodies (NAb) have been divided in 2 classes: overt and cryptic. Overt NAb can be detected in unfractionated normal sera of nonimmunized mammals and chickens. Cryptic NAb as described in mammals need an in vitro physical or biochemical treatment
Laure Diancourt et al.
Toxins, 11(3) (2019-03-03)
Clostridiumperfringens type E is a less frequently isolated C.perfringens type and has not previously been reported in France. We have characterized two recent type E isolates, C.perfringens 508.17 from the intestinal content of a calf that died of enterotoxemia, and
Zsuzsanna Kolbert et al.
Plant & cell physiology, 59(9), 1827-1843 (2018-05-26)
At high concentrations, selenium (Se) exerts phytotoxic effects in non-tolerant plant species partly due to the induction of nitro-oxidative stress; however, these processes are not fully understood. In order to obtain a more accurate view of the involvement of nitro-oxidative
Rheumatoid synovial fibroblasts constitutively express the fibrinolytic pattern of invasive tumor-like cells
Guiducci S, et al.
Clinical and Experimental Rheumatology, 23(3), 364-372 (2005)
Contrasting cellular stress responses of Baikalian and Palearctic amphipods upon exposure to humic substances: environmental implications
Protopopova MV, et al.
Environmental Science and Pollution Research International, 21(24), 14124-14137 (2014)
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Articles

Actin Protein

Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.

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