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Merck
CN

16951

Atto 495

BioReagent, suitable for fluorescence, ≥98.0% (HPCE)

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About This Item

UNSPSC Code:
12352200
PubChem Substance ID:
329751418
MDL number:
MFCD07370099

Key Documents

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Product Name

Atto 495, BioReagent, suitable for fluorescence, ≥98.0% (HPCE)

InChI

1S/C21H25N3O2.ClHO4/c1-22(2)17-9-7-15-12-16-8-10-18(23(3)4)14-20(16)24(19(15)13-17)11-5-6-21(25)26;2-1(3,4)5/h7-10,12-14H,5-6,11H2,1-4H3;(H,2,3,4,5)

SMILES string

[O-]Cl(=O)(=O)=O.CN(C)c1ccc2C=C3C=C\C(C=C3N(CCCC(O)=O)c2c1)=[N+](/C)C

InChI key

KCYUESOHZRSRGP-UHFFFAOYSA-N

product line

BioReagent

assay

≥98.0% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
DMF: soluble
DMSO: soluble
H2O: soluble
H2O: soluble
acetone: soluble
acetone: soluble

fluorescence

λex 499 nm; λem 526 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 495 is a lipophilic fluorescent compound, which has served as the neutral analyte in the model, along with SDS as the charged micelle acting as a carrier to transport this solute in an electric field. Atto 495 was included as fluorescence EOF marker for chip electrophoresis. This reagent is zwitterionic at pH 8.3 and thus migrates as a neutral substance under the proper conditions.
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

General description

Atto 495 is a new label for proteins and oligo-nucleotides with high molecular absorption (80.000) and quantum yield (0.45) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 495 is characterized by a high photostability and thermostability. It is slightly hydrophilic, and solubility in polar solvents as DMF or DMSO is high.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBT9606
1475740V

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Victor U Weiss et al.
Analytical chemistry, 82(19), 8146-8152 (2010-09-02)
Permeabilization of model lipid membranes by virus-derived peptides, viral proteins, and entire virions of human rhinovirus was assessed by quantifying the release of a fluorescent dye from liposomes via a novel chip electrophoretic assay. Liposomal leakage readily occurred upon incubation
Adolfo Téllez et al.
Electrophoresis, 30(2), 357-364 (2009-01-06)
The effect of formamide (FA) as a modifier on the retention in MEKC with SDS as the detergent was investigated. The mobility of a series of alkylphenones and of a zwitterionic fluorescent compound as a function of the FA and
Gerhard Bilek et al.
Electrophoresis, 30(24), 4292-4299 (2009-12-17)
In first attempts at elucidating the transfer of the RNA genome of a human Rhinovirus through lipid membranes in vitro we made use of liposomes decorated with recombinant receptors. This model system was characterized previously by CE but suffered from
Amos Bardea et al.
Analytical chemistry, 83(24), 9418-9423 (2011-11-02)
We report on a new ultrasensitive and fast technique for the detection and identification of both DNA and RNA with sensitivity of a few molecules. The new method is based on a patterned capillary tube (PCT) in which the internal
Adolfo Téllez et al.
Electrophoresis, 29(18), 3916-3923 (2008-10-14)
Three equilibria determine the interaction of a neutral analyte with the detergent in micellar electrokinetic chromatography and therefore its migration: (i) that of the free analyte in the aqueous phase with the micelle, (ii) its association with free detergent monomers
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