In modern pharmaceutical analysis, the utilization of Pharmacopeia regulated U/HPLC (Ultra/High-Performance Liquid Chromatography) methods has become a fundamental aspect of laboratory operations. This seminar aims to explore the diverse strategies and techniques that can be employed to fully leverage the potential of U/HPLC method adaptation, thereby enhancing the effectiveness and efficiency of laboratory processes. The presentation delves into the principles of U/HPLC and highlights the significance of adhering to Pharmacopeia regulations for ensuring reliable and accurate analytical results. Furthermore, various approaches for method adaptation, such as modification of mobile phase composition, adjustment of column parameters, and optimization of detection conditions will be discussed, including the benefits and challenges associated with each adaptation strategy, insights into the factors influencing method transfer and development. Additionally, the potential applications of U/HPLC method adaptation in pharmaceutical quality control, impurity profiling, and stability testing will be explored. By understanding the nuances and intricacies of U/HPLC method adaptation, laboratories can unlock new avenues for improving productivity, reducing costs, and enhancing the overall quality of pharmaceutical analysis.
Learning outcomes:
- Learn about the principles and importance of U/HPLC method adaptation in pharmaceutical analysis and how improved methods can help to boost laboratory effectiveness and efficiency.
- Gain insights into the significance of adhering to Pharmacopeia regulations for ensuring reliable and accurate analytical results.
- Understand various strategies and techniques for adapting U/HPLC methods, such as modifying mobile phase composition, and adjusting column parameters.
- Leverage the full potential of U/HPLC method adaptation leading to improved productivity and cost reduction in the laboratory.
Speakers
Petra Lewits
Merck
Global Product Manager for Analytical Chromatography, HPLC columns
Petra has worked with chromatography for 40 years. She started her career in separation science in 1983 using several chromatographic methods, including HPLC, TLC, GC and solid-phase extraction (SPE), for food control in a governmental institution lab. In 1986 she joined the pharmaceutical R&D and QC department (Merck KGaA, Darmstadt, Germany) where she developed HPLC methods for R&D and QC of pharmaceutical APIs and formulations as well as for diet convenience foods. From 1991 on she supported customers in different positions with technical and application-related inquires in Europe as a sales specialist for HPLC instruments, also providing technical support for chromatography and complaint management. Since 2002 she has been a Global Product Manager for Analytical Chromatography (HPLC). In 2014 she began focusing on thin-layer chromatography (TLC). Since April 2018 Petra has had global responsibility for analytical HPLC columns.
Egidijus Machtejevas
Merck
Sr. Technical Advisor, Analytical Chromatography Workflows
Egidijus Machtejevas was born in Kaunas, Lithuania where he studied chemistry, biotechnology and separation sciences (1991-2001) at Kaunas University of Technology, Lithuania. Also, as a research student spent a year in Prof. Stelan Hjerten laboratory in Uppsala University (Sweden). After gaining his PhD in analytical chemistry 2001-2007 he worked as a post-doc with Prof. Klaus Unger at Mainz University, Germany. Also two years spent at AstraZeneca (Sweden) where he set up and validated several various multidimensional systems for peptidomic analysis of different biological fluids. He joined the R&D Department at Merck KGaA, Darmstadt, Germany in 2008, in 2009 took a marketing manager position for North America, 2010 - 2018 product manager for chromatography. Currently, he is a Senior Technical Advisor in the Analytical Chromatography Workflows group. Egidijus Machtejevas has twenty-five scientific papers and thirteen book chapters to his name, and his major focus areas include chromatography, multidimensional liquid chromatography, proteomics and mass spectrometry.
Analytical chemistry
- Low pressure liquid chromatography
Duration:1h
Language:English
Session 1:presented October 17, 2023
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